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I have converted images to JPEG hopefully that will help. Please find attached images of glioblastoma case 2 images of microwave retrieval and 2 with pressure cooker, both using EDTA. Tried citrate buffer before and found similar problems. I have checked with the technicians and the case has low post mortem delay and fixation time
Asked on Apr 02 2012
Thank you for your email.
The CD51 and CD61 target is expressed by endothelial cells, megakaryocytes, monocytes, macrophages and Neutophils which is why the staining is more in the blood vessels.
It seems the antibody is binding to the cells where it suppose to bind however the staining is quite faint.
I suppose you are studying Glioblastoma in human brain which involves glial cells. Could I ask the reason of choosing CD51 and CD61 targets.
I will look forward to hearing from you soon.
Answered on Apr 02 2012