Overview

  • Product name

    Anti-Integrin alpha V beta 3 antibody [LM609]
    See all Integrin alpha V beta 3 primary antibodies
  • Description

    Mouse monoclonal [LM609] to Integrin alpha V beta 3
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Integrin alpha V beta 3.

  • Positive control

    • ICC/IF: Malme-3M cells. Flow Cytometry: HUVEC cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab190147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Relevance

    Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
  • Cellular localization

    Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
  • Database links

  • Alternative names

    • Antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD 61 antibody
    • CD51 antibody
    • CD51 antigen antibody
    • CD61 antibody
    • CD61 antigen antibody
    • DKFZp686A08142 antibody
    • GP3A antibody
    • GPIIIa antibody
    • Integrin alpha V antibody
    • Integrin alpha V vitronectin receptor alpha polypeptide antibody
    • Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • Integrin beta 3 antibody
    • Integrin beta 3 platelet glycoprotein IIIa antigen CD61 antibody
    • Integrin beta chain beta 3 antibody
    • integrinVbeta3 antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • MSK 8 antibody
    • MSK8 antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • Vitronectin receptor alpha polypeptide antibody
    • vitronectin receptor antibody
    • Vitronectin receptor subunit alpha antibody
    • VNR 1 antibody
    • VNR1 antibody
    • VNRA antibody
    see all

Images

  • Overlay histogram showing HUVEC (Human umbilical vein endothelial cell line) cells stained with ab190147 (red line). The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190147, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/4000 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab19353, 1 µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive result in 4% paraformaldehyde (10 minutes) fixed HUVEC cells used under the same conditions.

  • ab190147 stained Malme-3M cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab190147 at 5 µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

References

This product has been referenced in:

  • Wang Z  et al. Del1 Knockout Mice Developed More Severe Osteoarthritis Associated with Increased Susceptibility of Chondrocytes to Apoptosis. PLoS One 11:e0160684 (2016). Read more (PubMed: 27505251) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Blocking
Sample
Dog Cultured Cells (Ligament)
Specification
Ligament

Zara Smith

Verified customer

Submitted Sep 06 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 01 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate (10mM, pH 6.0)
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 23°C
Fixative
Paraformaldehyde

Ms. Noureen Ali

Verified customer

Submitted Mar 26 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 02 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC-3- prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
PC-3- prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Dec 29 2017

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