Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cells SkMel147)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Melanoma cells SkMel147
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 125, 130 kDa
Abcam user community
Verified customer
Submitted May 15 2017