Overview

  • Product name
    Anti-Integrin beta 1 antibody [BV7]
    See all Integrin beta 1 primary antibodies
  • Description
    Mouse monoclonal [BV7] to Integrin beta 1
  • Host species
    Mouse
  • Specificity
    This monoclonal antibody binds to human beta 1 integrin chain present on tumor cells. The antibody is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells, as well as several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.
  • Tested applications
    Suitable for: ELISA, WB, IP, Flow Cyt, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human umbilical vein EC.

  • Epitope
    Antibody BV7 stains the extracellular domain of beta-1 integrin.
  • Positive control
    • HT-29 colon carcinoma, Endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab7168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration. See Abreview.
Flow Cyt Use 1µg for 106 cells.

(paraformaldehyde or methanol fixed cells).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Inhibition Assay Use at an assay dependent concentration. The antibody has been functionally tested by adhesion assay, chemotaxis, patch clamp analysis and neurotoxicity induced apoptosis.

Target

  • Function
    Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
  • Tissue specificity
    Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    The cysteine residues are involved in intrachain disulfide bonds.
  • Cellular localization
    Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • beta1 integrin antibody
    • CD29 antibody
    • Fibronectin receptor subunit beta antibody
    • FNRB antibody
    • Glycoprotein IIa antibody
    • GP IIa antibody
    • GPIIA antibody
    • Integrin beta-1 antibody
    • Integrin subunit beta 1 antibody
    • integrin VLA-4 beta subunit antibody
    • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
    • ITB1_HUMAN antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • OTTHUMP00000019420 antibody
    • Very late activation protein, beta polypeptide antibody
    • VLA BETA antibody
    • VLA-4 subunit beta antibody
    • VLA-BETA antibody
    • VLAB antibody
    • VLAbeta antibody
    see all

Images

  • ab7168 at 2.5ug/ml immunoprecipitating integrin beta 1 from human OVCAR5 cell line whole cell lysate. OVCAR5 cell surface proteins were biotinylated at >95% confluence. Protein A/G was used for the immunoprecipitation and 5% milk was used as the blocking agent. Streptavidin-Ap was then used at 1/10000 to detect precipitated proteins.

    See Abreview

  • Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Overlay histogram showing HUVEC cells stained with ab7168 (blue line). Red line was isotype control mouse IgG1. Black line was an irrelevant mouse IgG1 used under the same conditions. Controls and anti-human ß1-integrin at 0.4 µg/100000 cells (1 hr 4°C).

References

This product has been referenced in:
  • Anguiano M  et al. Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis. PLoS One 12:e0171417 (2017). Read more (PubMed: 28166248) »
  • Jahangiri A  et al. Cross-activating c-Met/ß1 integrin complex drives metastasis and invasive resistance in cancer. Proc Natl Acad Sci U S A 114:E8685-E8694 (2017). Read more (PubMed: 28973887) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Answer

Thank you for contacting us.
We would recommend following antibodies for IP of integrin beta 1.
ab24693, can be used for IP of both human and mouse protein.
ab7168, ab30388, ab77803, and ab78502 can be used for IPing human beta 1 integrin protein and is fully guaranteed, we however haven't tested it with mouse protein so we are unsure about mouse cross reactivity. If you are interested in using one of these with mouse protein you may be interested in Abreview discount programme. Click the below link for more info;
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
ab95623 is used in IP with mouse lysates only and it is eligible for human IP collaboration discount.
Checkout the secondary antibody combinations;
ab7168 - ab96879
ab30388 - ab79091
ab95623- ab106783
For full list of secondary's click the link; https://www.abcam.com/index.html?pageconfig=productmap&cl=918
We do not have reagents for cell prtoein biotinylation. I can suggest the Invitrogen website;
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Biotin-Derivatives-and-Haptens/Biotinylation-and-Haptenylation-Reagents.html
If you are interested in Biotin conjugated antibodies then we have various biotinylation kits available. These are; https://www.abcam.com/index.html?pageconfig=resource&rid=13148

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thanks so much for forwarding the images. I am sorry to see that the results were so poor. Could you also send the general protocol that was used? As mentioned previously, I can send replacements or issue credit or refunds for the integrin beta 1 and integrin beta 3 antibodies, and I can issue a discount code to submit with an Abreview of the MOBP antibody in exchange for a free primary. We have several other antibodies to these targets that have been tested in human Western blot if you are interested in replacements. For integrin beta 1, our top-rated antibody is the mouse monoclonal ab30394 and our top-rate integrin beta 3 antibody is the mouse monoclonal ab7167- https://www.abcam.com/Integrin-beta-1-antibody-12G10-ab30394.html https://www.abcam.com/Integrin-beta-3-antibody-BV4-ab7167.html If you are interested in any of these options, please send me your Cedarlane customer code and full address so that I can make sure the replacement or refund is directed to you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Answer

Thanks for your reply and for clarifying that, and I look forward to receiving any data or images that you can provide. Please let me know if you need anything else from me.

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Answer

Thank you very much for your replies and for sending the information. I am sorry to hear that none of these antibodies gave the expected results. Just to clarify, there were no bands in Western blot even with the human protein samples? We do guarantee the integrin beta 1 antibody ab7168 and the integrin beta 3 antibody ab110131 to work in human samples, though the MOBP antibody ab66253 has not been tested with endogenous human samples. Could you also send some details about the Western blot protocol that was used, for our documentation? I would be happy to see if there are any suggestions that might improve the results. For ab7168 and ab110131, I can send replacement antibodies or issue credits if you would prefer. Could you also please send your Cedarlane customer number, so that I can ensure they pass on any credit or replacement to you? Thank you for your time, and I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

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Answer

Thank you for your inquiry and I'm sorry to hear that your customer is having trouble with these products. As for ab66253, we haven't tested this antibody in endogenous samples, so we are unsure how it will work with brain tissue.  However, we have tested both ab7168 and ab110131 to work with endogenous samples. What species were the brain tissues from?   Even the loading control seems a bit weak.  They may consider loading more protein than they currently are. We have heard that some customers have difficulty with our antibodies when using LiCor detection.  Does the customer have the ability to run it again using chromogenic/HRP detection? Typically what we've heard is that the Odyssey blocking buffer may inhibit binding.  If the customer can try blocking with 5% milk or BSA or 0.5% casein for 1 hr at RT instead of using the system's buffer, I would definitely recommend they try it. Also, they should decrease the time of washes to 3 x 5 mins instead of 10 mins. I hope this information helps.  Please contact us with any other questions or if the protocol suggestions do not improve the results.

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Answer

There is 92.5% homology between the Mouse and the Human integrin beta 1. The antibody ab7168 should therefore work with Mouse samples. DISCOUNT CODE: XXXXXXXXXX Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test ab7168 in Mouse. This code will give your customer 1 free primary antibody before the expiration date. To redeem this offer, please ask your customer to submit an Abreview for Mouse and to include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that customers can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (OVCAR5 cell line)
Total protein in input
50 µg
Specification
OVCAR5 cell line
Immuno-precipitation step
Protein A/G

Mr. John Andersen

Verified customer

Submitted Jul 21 2006

Question
Answer

Thank you for your phone call. I regret to inform you that concerning the antibodies that you are interested in, we do not have any information regarding the epitopes. If you have any more questions, please contact us again.

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Question
Answer

Since ab7168 is active on HT-29 colon carcinoma cells, HT 29 whole cell lysate (ab3952) may be a good choice.

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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