Anti-Integrin beta 1 antibody [BV7] (ab7168)

Reviews (1)Q&A (16)References (13)

Overview

  • Product name

    Anti-Integrin beta 1 antibody [BV7]
    See all Integrin beta 1 primary antibodies

  • Description

    Mouse monoclonal [BV7] to Integrin beta 1

  • Host species

    Mouse

  • Specificity

    This monoclonal antibody binds to human beta 1 integrin chain present on tumor cells. The antibody is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells, as well as several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.

  • Tested applications

    Suitable for: ELISA, WB, IP, Flow Cyt, Inhibition Assaymore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Human umbilical vein EC.

  • Epitope

    Antibody BV7 stains the extracellular domain of beta-1 integrin.

  • Positive control

    • HT-29 colon carcinoma, Endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab7168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration. See Abreview.
Flow Cyt Use 1µg for 106 cells.

(paraformaldehyde or methanol fixed cells).

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. The antibody has been functionally tested by adhesion assay, chemotaxis, patch clamp analysis and neurotoxicity induced apoptosis.

Target

  • Function

    Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).

  • Tissue specificity

    Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).

  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.

  • Post-translational
    modifications

    The cysteine residues are involved in intrachain disulfide bonds.

  • Cellular localization

    Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Information by UniProt

  • Database links

    • Alternative names

      • beta1 integrin antibody
      • CD_antigen=CD29 antibody
      • CD29 antibody
      • Fibronectin receptor subunit beta antibody
      • FNRB antibody
      • Glycoprotein IIa antibody
      • GP IIa antibody
      • GPIIA antibody
      • Integrin beta-1 antibody
      • Integrin subunit beta 1 antibody
      • integrin VLA-4 beta subunit antibody
      • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
      • ITB1_HUMAN antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • OTTHUMP00000019420 antibody
      • very late activation protein antibody
      • Very late activation protein, beta polypeptide antibody
      • VLA BETA antibody
      • VLA-4 subunit beta antibody
      • VLA-BETA antibody
      • VLAB antibody
      • VLAbeta antibody
      see all

    Images

    • Immunoprecipitation - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Immunoprecipitation - Anti-Integrin beta 1 antibody [BV7] (ab7168)This image is courtesy of an Abreview submitted by Mr John Andersen
      ab7168 at 2.5ug/ml immunoprecipitating integrin beta 1 from human OVCAR5 cell line whole cell lysate. OVCAR5 cell surface proteins were biotinylated at >95% confluence. Protein A/G was used for the immunoprecipitation and 5% milk was used as the blocking agent. Streptavidin-Ap was then used at 1/10000 to detect precipitated proteins.

      See Abreview

    • Flow Cytometry - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Flow Cytometry - Anti-Integrin beta 1 antibody [BV7] (ab7168)

      Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

      Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    • Flow Cytometry - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Flow Cytometry - Anti-Integrin beta 1 antibody [BV7] (ab7168)
      Overlay histogram showing HUVEC cells stained with ab7168 (blue line). Red line was isotype control mouse IgG1. Black line was an irrelevant mouse IgG1 used under the same conditions. Controls and anti-human ß1-integrin at 0.4 µg/100000 cells (1 hr 4°C).

    References

    This product has been referenced in:

    • Fujita M  et al. Autocrine Production of PDGF Stimulated by the Tenascin-C-Derived Peptide TNIIIA2 Induces Hyper-Proliferation in Glioblastoma Cells. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 31261783) »
    • Anguiano M  et al. Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis. PLoS One 12:e0171417 (2017). Read more (PubMed: 28166248) »
    See all 13 Publications for this product

    Publishing research using ab7168? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-10 of 17 Abreviews or Q&A

    Question

    I would like to immunoprecipitate beta1 integrin from mouse and human cells. In some cases I want to biotinylate the surface proteins prior to preparing a cellular lysate and immunoprecipitation.
    Can you suggest an antibody that would be useful?
    Thank you,

    Read More
    Answer

    Thank you for contacting us.
    We would recommend following antibodies for IP of integrin beta 1.
    ab24693, can be used for IP of both human and mouse protein.
    ab7168, ab30388, ab77803, and ab78502 can be used for IPing human beta 1 integrin protein and is fully guaranteed, we however haven't tested it with mouse protein so we are unsure about mouse cross reactivity. If you are interested in using one of these with mouse protein you may be interested in Abreview discount programme. Click the below link for more info;
    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
    ab95623 is used in IP with mouse lysates only and it is eligible for human IP collaboration discount.
    Checkout the secondary antibody combinations;
    ab7168 - ab96879
    ab30388 - ab79091
    ab95623- ab106783
    For full list of secondary's click the link; https://www.abcam.com/index.html?pageconfig=productmap&cl=918
    We do not have reagents for cell prtoein biotinylation. I can suggest the Invitrogen website;
    http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Biotin-Derivatives-and-Haptens/Biotinylation-and-Haptenylation-Reagents.html
    If you are interested in Biotin conjugated antibodies then we have various biotinylation kits available. These are; https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    We use homogenized human brain tissue to isolate the protein.  We run the protein on 4-20% gels. We used PVDF membrane for the transfer.  We use licor-odyssey blocking buffer as our blocking agent for 1 hour at room temperature.  We used a concentration of 1:1000 for all primary antibodies.  We incubated in primary antibody overnight at 4 degrees C. We washed 3 times for 10 minutes in PBS-Tween then incubated in Goat-anti-mouse secondary antibody from licor-odyssey for 1 hour at room temperature.  We then washed 3 times for 10 minutes  with PBS-Tween and used a Licor-Odyssey Infrared Imager to detect the proteins.   I think we would like credits issued for the Integrin beta 1 and 3.

    Read More
    Answer

    Thank you very much for sending this information. I am sorry that these antibodies did not work as expected. I have issued the credits to Cedarlane, and I will contact them to pass these credits on to you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Question

    Attached are Images from the antibodies on Human samples.  

    Read More
    Answer

    Thanks so much for forwarding the images. I am sorry to see that the results were so poor. Could you also send the general protocol that was used? As mentioned previously, I can send replacements or issue credit or refunds for the integrin beta 1 and integrin beta 3 antibodies, and I can issue a discount code to submit with an Abreview of the MOBP antibody in exchange for a free primary. We have several other antibodies to these targets that have been tested in human Western blot if you are interested in replacements. For integrin beta 1, our top-rated antibody is the mouse monoclonal ab30394 and our top-rate integrin beta 3 antibody is the mouse monoclonal ab7167- https://www.abcam.com/Integrin-beta-1-antibody-12G10-ab30394.html https://www.abcam.com/Integrin-beta-3-antibody-BV4-ab7167.html If you are interested in any of these options, please send me your Cedarlane customer code and full address so that I can make sure the replacement or refund is directed to you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Question

    Thanks for the quick reply. There were no bands with those antibodies which is something that we rarely find. We ran the blots with other antibodies (psd95) and got the typical bands.   

    Read More
    Answer

    Thanks for your reply and for clarifying that, and I look forward to receiving any data or images that you can provide. Please let me know if you need anything else from me.

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    Question

    We ordered 3 antibodies.  They were MOBP AB66253, Integrin beta 1 AB7168 and Integrin beta 3 AB110131. We used western blot on Human protein samples, Rat protein samples, rabbit protein samples and cat protein samples. We did not see any bands in either homogenate or synaptoneurosome preparations.   We ordered these antibodies through cedarlane  

    Read More
    Answer

    Thank you very much for your replies and for sending the information. I am sorry to hear that none of these antibodies gave the expected results. Just to clarify, there were no bands in Western blot even with the human protein samples? We do guarantee the integrin beta 1 antibody ab7168 and the integrin beta 3 antibody ab110131 to work in human samples, though the MOBP antibody ab66253 has not been tested with endogenous human samples. Could you also send some details about the Western blot protocol that was used, for our documentation? I would be happy to see if there are any suggestions that might improve the results. For ab7168 and ab110131, I can send replacement antibodies or issue credits if you would prefer. Could you also please send your Cedarlane customer number, so that I can ensure they pass on any credit or replacement to you? Thank you for your time, and I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Question

    I had a customer contact me regarding some problems she had with three antibodies ordered on PO 134004. I have attached her protocol information and images. She had also ordered AB62631 at the same time, and this antibody worked well for her.

    Read More
    Answer

    Thank you for your inquiry and I'm sorry to hear that your customer is having trouble with these products. As for ab66253, we haven't tested this antibody in endogenous samples, so we are unsure how it will work with brain tissue.  However, we have tested both ab7168 and ab110131 to work with endogenous samples. What species were the brain tissues from?   Even the loading control seems a bit weak.  They may consider loading more protein than they currently are. We have heard that some customers have difficulty with our antibodies when using LiCor detection.  Does the customer have the ability to run it again using chromogenic/HRP detection? Typically what we've heard is that the Odyssey blocking buffer may inhibit binding.  If the customer can try blocking with 5% milk or BSA or 0.5% casein for 1 hr at RT instead of using the system's buffer, I would definitely recommend they try it. Also, they should decrease the time of washes to 3 x 5 mins instead of 10 mins. I hope this information helps.  Please contact us with any other questions or if the protocol suggestions do not improve the results.

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    Question

    Dear technical team,   Our customer would like to purchase anti-integrin beta 1 antiody for inhibition on mouse sample.   There's no product for mouse sample so, I would like to know it is possible to test with discount code.   Check this please.  

    Read More
    Answer

    There is 92.5% homology between the Mouse and the Human integrin beta 1. The antibody ab7168 should therefore work with Mouse samples. DISCOUNT CODE: XXXXXXXXXX Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test ab7168 in Mouse. This code will give your customer 1 free primary antibody before the expiration date. To redeem this offer, please ask your customer to submit an Abreview for Mouse and to include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that customers can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Immunoprecipitation abreview for Anti-Integrin beta 1 antibody [BV7]

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (OVCAR5 cell line)
    Total protein in input
    50 µg
    Specification
    OVCAR5 cell line
    Immuno-precipitation step
    Protein A/G
    Read More

    Mr. John Andersen

    Verified customer

    Submitted Jul 21 2006

    Question

    Customer would like to know: what end of the integrin was the antibody raised against?

    Read More
    Answer

    Thank you for your phone call. I regret to inform you that concerning the antibodies that you are interested in, we do not have any information regarding the epitopes. If you have any more questions, please contact us again.

    Read More

    Question

    What do you recommend as a positive control?

    Read More
    Answer

    Since ab7168 is active on HT-29 colon carcinoma cells, HT 29 whole cell lysate (ab3952) may be a good choice.

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    1-10 of 17 Abreviews or Q&A

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