Anti-Integrin beta 1 antibody [BV7] (ab7168)

Thank you for contacting us.
We would recommend following antibodies for IP of integrin beta 1.
ab24693, can be used for IP of both human and mouse protein.
ab7168, ab30388, ab77803, and ab78502 can be used for IPing human beta 1 integrin protein and is fully guaranteed, we however haven't tested it with mouse protein so we are unsure about mouse cross reactivity. If you are interested in using one of these with mouse protein you may be interested in Abreview discount programme. Click the below link for more info;
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
ab95623 is used in IP with mouse lysates only and it is eligible for human IP collaboration discount.
Checkout the secondary antibody combinations;
ab7168 - ab96879
ab30388 - ab79091
ab95623- ab106783
For full list of secondary's click the link; https://www.abcam.com/index.html?pageconfig=productmap&cl=918
We do not have reagents for cell prtoein biotinylation. I can suggest the Invitrogen website;
http://www.invitrogen.com/site/us/en/home/References/Molecular-Probes-The-Handbook/Biotin-Derivatives-and-Haptens/Biotinylation-and-Haptenylation-Reagents.html
If you are interested in Biotin conjugated antibodies then we have various biotinylation kits available. These are; https://www.abcam.com/index.html?pageconfig=resource&rid=13148

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for sending this information. I am sorry that these antibodies did not work as expected. I have issued the credits to Cedarlane, and I will contact them to pass these credits on to you. Please let me know if you have any questions or if there is anything else that we can do for you.

Thanks so much for forwarding the images. I am sorry to see that the results were so poor. Could you also send the general protocol that was used? As mentioned previously, I can send replacements or issue credit or refunds for the integrin beta 1 and integrin beta 3 antibodies, and I can issue a discount code to submit with an Abreview of the MOBP antibody in exchange for a free primary. We have several other antibodies to these targets that have been tested in human Western blot if you are interested in replacements. For integrin beta 1, our top-rated antibody is the mouse monoclonal ab30394 and our top-rate integrin beta 3 antibody is the mouse monoclonal ab7167- https://www.abcam.com/Integrin-beta-1-antibody-12G10-ab30394.html https://www.abcam.com/Integrin-beta-3-antibody-BV4-ab7167.html If you are interested in any of these options, please send me your Cedarlane customer code and full address so that I can make sure the replacement or refund is directed to you. Please let me know if you have any questions or if there is anything else that we can do for you.

Thanks for your reply and for clarifying that, and I look forward to receiving any data or images that you can provide. Please let me know if you need anything else from me.

Thank you very much for your replies and for sending the information. I am sorry to hear that none of these antibodies gave the expected results. Just to clarify, there were no bands in Western blot even with the human protein samples? We do guarantee the integrin beta 1 antibody ab7168 and the integrin beta 3 antibody ab110131 to work in human samples, though the MOBP antibody ab66253 has not been tested with endogenous human samples. Could you also send some details about the Western blot protocol that was used, for our documentation? I would be happy to see if there are any suggestions that might improve the results. For ab7168 and ab110131, I can send replacement antibodies or issue credits if you would prefer. Could you also please send your Cedarlane customer number, so that I can ensure they pass on any credit or replacement to you? Thank you for your time, and I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you for your inquiry and I'm sorry to hear that your customer is having trouble with these products. As for ab66253, we haven't tested this antibody in endogenous samples, so we are unsure how it will work with brain tissue.  However, we have tested both ab7168 and ab110131 to work with endogenous samples. What species were the brain tissues from?   Even the loading control seems a bit weak.  They may consider loading more protein than they currently are. We have heard that some customers have difficulty with our antibodies when using LiCor detection.  Does the customer have the ability to run it again using chromogenic/HRP detection? Typically what we've heard is that the Odyssey blocking buffer may inhibit binding.  If the customer can try blocking with 5% milk or BSA or 0.5% casein for 1 hr at RT instead of using the system's buffer, I would definitely recommend they try it. Also, they should decrease the time of washes to 3 x 5 mins instead of 10 mins. I hope this information helps.  Please contact us with any other questions or if the protocol suggestions do not improve the results.

There is 92.5% homology between the Mouse and the Human integrin beta 1. The antibody ab7168 should therefore work with Mouse samples. DISCOUNT CODE: XXXXXXXXXX Expiration date: DD MM YYYY I am very pleased to hear your customer would like to accept our offer and test ab7168 in Mouse. This code will give your customer 1 free primary antibody before the expiration date. To redeem this offer, please ask your customer to submit an Abreview for Mouse and to include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that customers can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for your phone call. I regret to inform you that concerning the antibodies that you are interested in, we do not have any information regarding the epitopes. If you have any more questions, please contact us again.

Since ab7168 is active on HT-29 colon carcinoma cells, HT 29 whole cell lysate (ab3952) may be a good choice.