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  1. Link

    integrin-beta-1-antibody-ep1041y-ab52971.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

  • Datasheet
  • SDS
Reviews (22)Q&A (11)References (109)

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Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
  • Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1041Y] to Integrin beta 1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Integrin beta 1 antibody [EP1041Y]
    See all Integrin beta 1 primary antibodies
  • Description

    Rabbit monoclonal [EP1041Y] to Integrin beta 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Integrin beta 1 aa 650-750. The exact sequence is proprietary.
    Database link: P05556

  • Positive control

    • WB: HeLa, HT-1080, U937, U87-MG and U2OS whole cell lysates. IHC-P: Human transitional cell carcinoma of bladder and human breast cancer metastasis tissues; Human lung tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.1% BSA, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1041Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

Associated products

  • Alternative Versions

    • Anti-Integrin beta 1 antibody [EP1041Y] - BSA and Azide free (ab192456)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Integrin beta 1 protein (ab114157)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (10)
1/1000 - 1/100000. Detects a band of approximately 140-150 kDa (predicted molecular weight: 88 kDa).

For unpurified, use 1/500.

IHC-P (5)
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Use of an HRP/AP polymerized secondary antibody is recommended.

For unpurified, use 1/250 - 1/500.

Notes
WB
1/1000 - 1/100000. Detects a band of approximately 140-150 kDa (predicted molecular weight: 88 kDa).

For unpurified, use 1/500.

IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Use of an HRP/AP polymerized secondary antibody is recommended.

For unpurified, use 1/250 - 1/500.

Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
    Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
    (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733).
  • Tissue specificity

    Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    The cysteine residues are involved in intrachain disulfide bonds.
  • Cellular localization

    Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner.
  • Target information above from: UniProt accession P05556 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3688 Human
    • Omim: 135630 Human
    • SwissProt: P05556 Human
    • Unigene: 643813 Human
    • Alternative names

      • beta1 integrin antibody
      • CD_antigen=CD29 antibody
      • CD29 antibody
      • Fibronectin receptor subunit beta antibody
      • FNRB antibody
      • Glycoprotein IIa antibody
      • GP IIa antibody
      • GPIIA antibody
      • Integrin beta-1 antibody
      • Integrin subunit beta 1 antibody
      • integrin VLA-4 beta subunit antibody
      • Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody
      • ITB1_HUMAN antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • OTTHUMP00000019420 antibody
      • very late activation protein antibody
      • Very late activation protein, beta polypeptide antibody
      • VLA BETA antibody
      • VLA-4 subunit beta antibody
      • VLA-BETA antibody
      • VLAB antibody
      • VLAbeta antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 6 and 10: Integrin beta 1 knockout HAP1 cell lysate (20 µg)
      Lanes 3, 7 and 11: U87-MG cell lysate (20 µg)
      Lanes 4, 8 and 12: A431 cell lysate (20 µg)
      Lanes 1, 2, 3 and 4: Green signal from target – ab52971 observed at 140 kDa
      Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab52971 was shown to specifically react with Integrin beta 1 in wild-type HAP1 cells. No band was observed when Integrin beta 1 knockout samples were examined. Wild-type and Integrin beta 1 knockout samples were subjected to SDS-PAGE. ab52971 and ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

      ab52971 at 1/500 staining Integrin beta 1 antibody in human transitional cell carcinoma of bladder by immunohistochemistry (FFPE).

      Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin beta 1 with ab52971 at 1/500 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Integrin beta 1 knockout HAP1 cell lysate (20 µg)
      Lane 3: U87-MG cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab52971 observed at 140 kDa. Red signal from loading control – ab8245 observed at 37 kDa.

      This western blot image is a comparison between ab52971 and a competitor's top cited rabbit polyclonal antibody.

    • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      All lanes : Anti-Integrin beta 1 antibody [EP1041Y] (ab52971) at 1/10000 dilution (purified)

      Lane 1 : U87-MG cell lysate
      Lane 2 : HT-1080 cell lysate
      Lane 3 : U937 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 88 kDa
      Observed band size: 140 kDa why is the actual band size different from the predicted?



      5% NFDM/TBST dilution buffer

    • Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Western blot - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      All lanes : Anti-Integrin beta 1 antibody [EP1041Y] (ab52971) at 20 µg (unpurified)

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
      Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

      Predicted band size: 88 kDa
      Observed band size: 120 + 140 kDa why is the actual band size different from the predicted?



      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab52971 overnight at 4°C. Antibody binding was detected using ab175781 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

       

      Secondary antibody - anti-rabbit Alexa Fluor 790

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)This image is courtesy of an anonymous Abreview

      Unpurified ab52971 staining human breast cancer metastasis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with a commercial blocking reagent and incubation with the antibody (diluted 1/100) for 18 hours at 4°C.  A HRP-conjugated goat anti-rabbit was used as the secondary antibody.  This image shows a cancer metastasis at 40x with beta1 staining (in red) in both blood vessels and tumour cells. Blue is Hoechst for nuclei.  The antibody detection was enhanced using a commercial Cy3 tyramide signal amplification kit.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)This image is courtesy of an Abreview submitted by Dr. Mark Southwood.

      Formaldehyde-fixed, paraffin-embedded human lung tissue stained for Integrin beta 1 using ab52971 at 1/100 dilution in immunohistochemical analysis.

      See Abreview

    • Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)
      Anti-Integrin beta 1 antibody [EP1041Y] (ab52971)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (109)

    Publishing research using ab52971? Please let us know so that we can cite the reference in this datasheet.

    ab52971 has been referenced in 109 publications.

    • Liu Z  et al. Downregulation of miR-29b-3p promotes a-tubulin deacetylation by targeting the interaction of matrix metalloproteinase-9 with integrin ß1 in nasal polyps. Int J Mol Med 48:N/A (2021). PubMed: 33982786
    • Zhu J  et al. CD151 drives cancer progression depending on integrin a3ß1 through EGFR signaling in non-small cell lung cancer. J Exp Clin Cancer Res 40:192 (2021). PubMed: 34108040
    • Rohn F  et al. Impaired integrin a5 /ß1 -mediated hepatocyte growth factor release by stellate cells of the aged liver. Aging Cell 19:e13131 (2020). PubMed: 32157808
    • Kim SE  et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510
    • Wang J  et al. The proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma. Pulm Pharmacol Ther 63:101931 (2020). PubMed: 32818548
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 33 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Medaka fish Tissue sections (Basal side of epithelia)
    Specification
    Basal side of epithelia
    Fixative
    DMSO+Methanol
    Permeabilization
    Yes - acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Makoto Furutani-Seiki

    Verified customer

    Submitted Nov 15 2011

    Western blot abreview for Anti-Integrin beta 1 antibody [EP1041Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cancer cell)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    50 µg
    Treatment
    KSRP mutants transfection
    Specification
    colon cancer cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 16 2020

    Western blot abreview for Anti-Integrin beta 1 antibody [EP1041Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    30 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 21 2019

    Western blot abreview for Anti-Integrin beta 1 antibody [EP1041Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (melanoma cell lines)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    melanoma cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Praveen Agrawal

    Verified customer

    Submitted Mar 24 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin beta 1 antibody [EP1041Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Primary humain airway epithelial cells)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate biffer 10mM
    Permeabilization
    No
    Specification
    Primary humain airway epithelial cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Aaron Gardner

    Verified customer

    Submitted Nov 30 2016

    Western blot abreview for Anti-Integrin beta 1 antibody [EP1041Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Sample
    Human Cell lysate - whole cell (non small cell lung cancer)
    Specification
    non small cell lung cancer
    Treatment
    siRNA treated
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Oct 07 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin beta 1 antibody [EP1041Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample
    Human Tissue sections (Infantile fibromatosis)
    Specification
    Infantile fibromatosis
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 21 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    1% serum + 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
    Sample
    Mouse Cell (mouse embryonic stem cells)
    Specification
    mouse embryonic stem cells
    Permeabilization
    Yes - 0.1% triton X
    Fixative
    methanol:acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 30 2014

    Western blot abreview for Anti-Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1e+006 cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification
    mouse embryonic stem cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 28 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end

    Below Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Sample
    Human Cell (HeLa cell)
    Specification
    HeLa cell
    Permeabilization
    Yes - 0.5% TritonX/PBS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 19 2013

    1-10 of 33 Abreviews or Q&A

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