Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Normal bladder)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer
Permeabilization
No
Specification
Normal bladder
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS
Dilution
1/500
Additional data
Additional Notes
Blocking in PBS + 10% Normal Goat Serum (ab7481)
Tris-EDTA buffer recipe as described in https://www.abcam.com/protocols/ihc-antigen-retrieval-protocol
Heat mediated antigen retrieval: 20 min in a steamer
After DAB, counter stained with hematoxylin.
Beta 1 expression in the dog bladder tissue was detected using ab179471.
Secondary Ab only control was also conducted (data not shown).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Mar 15 2016