Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16F10(melanoma) syngeneic mouse tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
B16F10(melanoma) syngeneic mouse tumor
Fixative
Formaldehyde
Other product details
Incubation time
36 minute(s) · Temperature: 37°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rb-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
I think that the primary antibody concentration (1:1000) was too high in this staining condition.
The border lines of cells were well distinguished.
It would be better to dilute the primary antibody more to reduce background staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Dec 20 2021