Anti-Integrin beta 1d antibody [2B1] (ab8991)

Submit a review Q&A (6)References (5)
Western blot - Anti-Integrin beta 1d antibody [2B1] (ab8991)
  • Immunocytochemistry - Anti-Integrin beta 1d antibody [2B1] (ab8991)

Key features and details

  • Mouse monoclonal [2B1] to Integrin beta 1d
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Integrin beta 1d antibody [2B1]

  • Description

    Mouse monoclonal [2B1] to Integrin beta 1d

  • Host species

    Mouse

  • Specificity

    Recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.

    A broad species reactivity is expected because of the conserved nature of the epitope.

  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Integrin beta 1d. The immunogen corresponds to the C-terminal 24 amino acids of integrin ß1D including an appending N-terminal cysteine (

    CQENPIYKS-PINNFKNPNYGRKAGL

    ) coupled to keyhole limpet hemocyanin.
    Database link: P05556
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    Cytoplasmic domain, C-terminal. Isoform of the Integrin b1 subunit present in cardiac and skeletal muscle.

  • Positive control

    • WB: Human heart, tongue and skeletal muscle tissue lysates. ICC: Human myocardium.

  • General notes


    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Primary antibody notes

    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.

  • Clonality

    Monoclonal

  • Clone number

    2B1

  • Myeloma

    Sp2/0

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
WB
Human
Application Abreviews Notes
ICC
1/25 - 1/200.
WB
1/100 - 1/1000.
Notes
ICC
1/25 - 1/200.
WB
1/100 - 1/1000.

Target

  • Relevance

    Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. The isoform beta-1D of Integrin 1, is expressed specifically in striated muscle (skeletal and cardiac muscle).

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • CD29 antibody
      • FNRB antibody
      • Integrin beta 1 antibody
      • ITGB 1 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 1d antibody [2B1] (ab8991)
      Western blot - Anti-Integrin beta 1d antibody [2B1] (ab8991)

      Western blotting of beta1D integrin showing its muscle cell specific expression pattern.

    • Immunocytochemistry - Anti-Integrin beta 1d antibody [2B1] (ab8991)
      Immunocytochemistry - Anti-Integrin beta 1d antibody [2B1] (ab8991)

      Immunofluorescence staining of myocardium for beta1D integrin.

    References (5)

    Publishing research using ab8991? Please let us know so that we can cite the reference in this datasheet.

    ab8991 has been referenced in 5 publications.

    • Yuan T  et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). PubMed: 30070011
    • Lee HJ  et al. Dysregulation of nuclear receptor COUP-TFII impairs skeletal muscle development. Sci Rep 7:3136 (2017). WB, IF ; Mouse . PubMed: 28600496
    • Barton ER  et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J : (2012). ICC/IF ; Mouse . PubMed: 22649033
    • Barton ER Restoration of gamma-sarcoglycan localization and mechanical signal transduction are independent in murine skeletal muscle. J Biol Chem 285:17263-70 (2010). WB ; Mouse . PubMed: 20371873
    • Evans M  et al. Expression profiling reveals heightened apoptosis and supports fiber size economy in the murine muscles of mastication. Physiol Genomics 35:86-95 (2008). PubMed: 18593863

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Question

    Do yu have any other cardiac surface marker aprt from from integrin 1beta which reacts with mouse tissue?


    Thanks

    Read More
    Answer

    Please forward the following publication to customer, there are many markers listed and we have antibodies against these. Customer might need to make decision based on their requirements.

    http://journals.tums.ac.ir/upload_files/pdf/_/20047.pdf

    The cardiac cell surface markers are like; KCNA6; https://www.abcam.com/kv16-antibody-ab21089.html

    https://www.abcam.com/popdc2-antibody-ab69176.html

    β1- and β2-adrenergic receptors etc.

    Read More

    Question

    Hello, I want to have an antibodiy which identifies Striated muscles(skeletal muscles) surfaced markers. Please advise me.

    Read More
    Answer


    I would recommend to use an anti-integrin beta 1d such as https://www.abcam.com/ab8992 or https://www.abcam.com/ab8991.

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    Question

    Abcam Technical Help, We recently purchased Integrin antibody (ab8991), and we tried to do immunohitology on skeletal muscle tissue with suggested dilutions. Yet, we did not get a plausible result. So, we would like to know staining conditions for the myocardium (ex. blocking condition, first and second antibodies conditions). If that is not available through abcam, may I contact the source of the image? Thank you for helping us out.

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    Answer

    Thank you for your enquiry. I am sorry it has taken a little time to obtain this protocol for you. Thank you for your patience. I hope this will be helpflu to you. Please note that this procedure would be a guideline only and may require some further optmization in your laboratory. Indirect immunofluorescence protocol for ab8991 Integrin beta 1d antibody [2B1]: 1. Cut 5µm sections of the frozen tissue with a thickness of 5 µm. 2. Allow them to dry a few minutes at room temperature. 3. Fixation: 20 min in aceton at -20°C, make sure the aceton is pre-cooled. 4. Wash 3 times for 5 min with PBS on a shaker. 5. Dissolve the primary antibody in PBS/ 0,1 % BSA according to the instructions in the datasheet. 6. Add minimally 100 µl diluted antibody to the tissue section. Put the slides in a closed box with wet tissues. It will keep the humidity high and prevents drying of the antibody solution. 7. Incubate the slides 60 minutes at 37°C. 8. Rinse the slides PBS. 9. Wash 3 times for 5 min with PBS on a shaker. Always use a different jar per antibody and wash the negative control separately. 10. Dilution the secondary FITC conjugated antibody (rabbit anti-mouse) 1:100 in PBS/ 0.1 % BSA. 11. Add minimally 100 µl diluted secondary antibody to the tissue section. Put again the slides in a closed box with wet tissues. 12. Incubate the slides 30 minutes at 37°C 13. Wash 3 times for 5 min with PBS on a shaker 14. Dehydrate the slides in an ascending ethanol series, 70%, 96% and 100%. 15. Allow the slides to dry. It is important that the tissue section is completely dry. 16. Mount slides with 0.5 µg DAPI/ml in the mounting medium (50% glycerol in 0.02 M TRIS HCl; pH7.4, containing 2% 1,4-diazobicyclo(2,2,2)-octane (DABCO)). 17. Absorb the excess of mounting medium. 18. Use nail polish to adhere the cover glass to the slides. 19. Observe in fluorescence microscope. If you have any further questions, please do not hesitate to contact us.

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    Question

    BATCH NUMBER 96533 ORDER NUMBER BH5540 DESCRIPTION OF THE PROBLEM No signal SAMPLE human skeletal muscle extract from BioChain PRIMARY ANTIBODY ab8991 anti beta 1D integrin from ABCAM, 1/1000 or 1/200 over night at 4?C Wash in PBS 0,05% tween SECONDARY ANTIBODY Goat anti mouse fom Upstate conditions validated by the highly positive signal given by anti troponin I ABCAM AB8294 DETECTION METHOD Immun star HRP from biorad POSITIVE AND NEGATIVE CONTROLS USED human skeletal muscle extract from BioChain with anti troponin I ABCAM AB8294 ANTIBODY STORAGE CONDITIONS 4?C SAMPLE PREPARATION Biochain prep conditions include protease inhibitor. Sample reconstituted in Laemmli buffer plus DTT heated 5mn at 95?C. AMOUNT OF PROTEIN LOADED 25 ?g ELECTROPHORESIS/GEL CONDITIONS reducing bis tris XT criterion gel 4-12% from Biorad TRANSFER AND BLOCKING CONDITIONS Transfer on PVDF Blocking agent : milk 10% in PBS buffer 0,05% tween. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Concentration of the primary antibody 1/1000 and 1/200

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    Answer

    We have just received the following information from the source of ab8991: "We would suggest to increase the protein concentration in the gel and to use more concentrated antibody for the blotting as this antibody is supplied as tissue culture supernatant." I apologise for the delay in replying to you, please be assured that if you still have a problem we will send you a replacement or refund if you purchased the antibody in the last 90 days.

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    Question

    What are the recommended dilutions on this antibody for Westerns and immunohistochemistry

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    Answer

    Thank you for your enquiry. For Western blotting, I would suggest starting at a dilution of 1:1000. For IHC, I would recommend titrating the antibody 1:20 to 1:200. For both applications, you will need to optimize based upon results. Please contact us again if you have any additional questions.

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    Question

    Can I use this antibody to test rat or mice tissues?

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    Answer

    We would expect this antibody to work on rat and mouse tissues, but cannot guarantee it. This epitope is conserved across species.

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