Overview

  • Product name
    Anti-Integrin beta 1d antibody [2B1]
  • Description
    Mouse monoclonal [2B1] to Integrin beta 1d
  • Host species
    Mouse
  • Specificity
    Recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: C-QENPIYKSPINNFKNPNYGRKAGL conjugated to KLH.

  • Epitope
    Cytoplasmic domain, C-terminal. Isoform of the Integrin b1 subunit present in cardiac and skeletal muscle.
  • General notes


    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.
  • Clonality
    Monoclonal
  • Clone number
    2B1
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000.
IHC-Fr 1/25 - 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. The isoform beta-1D of Integrin 1, is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • Cellular localization
    Cell Membrane
  • Database links
    • Alternative names
      • CD29 antibody
      • FNRB antibody
      • Integrin beta 1 antibody
      • ITGB 1 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 1d antibody [2B1] (ab8991)
      Western blot - Anti-Integrin beta 1d antibody [2B1] (ab8991)

      Western blotting of beta1D integrin showing its muscle cell specific expression pattern.

      Western blotting of beta1D integrin showing its muscle cell specific expression pattern.
    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1d antibody [2B1] (ab8991)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1d antibody [2B1] (ab8991)

      Immunofluorescence staining of myocardium for beta1D integrin.

    References

    This product has been referenced in:
    • Lee HJ  et al. Dysregulation of nuclear receptor COUP-TFII impairs skeletal muscle development. Sci Rep 7:3136 (2017). WB, IF ; Mouse . Read more (PubMed: 28600496) »
    • Barton ER  et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J : (2012). ICC/IF ; Mouse . Read more (PubMed: 22649033) »

    See all 4 Publications for this product

    Publishing research using ab8991? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    6 reviews or Q&A

    10
    Votes: Highest First

    Do yu have any other cardiac surface marker aprt from from integrin 1beta which reacts with mouse tissue?


    Thanks

    Please forward the following publication to customer, there are many markers listed and we have antibodies against these. Customer might need to make decision based on their requirements.

    http://journals.tums.ac.ir/upload_files/pdf/_/20047.pdf...

    Read More

    Hello, I want to have an antibodiy which identifies Striated muscles(skeletal muscles) surfaced markers. Please advise me.


    I would recommend to use an anti-integrin beta 1d such as https://www.abcam.com/ab8992 or https://www.abcam.com/ab8991.

    Abcam Technical Help, We recently purchased Integrin antibody (ab8991), and we tried to do immunohitology on skeletal muscle tissue with suggested dilutions. Yet, we did not get a plausible result. So, we would like to know staining conditions fo...

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    Thank you for your enquiry. I am sorry it has taken a little time to obtain this protocol for you. Thank you for your patience. I hope this will be helpflu to you. Please note that this procedure would be a guideline only and may require some fur...

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    BATCH NUMBER 96533 ORDER NUMBER BH5540 DESCRIPTION OF THE PROBLEM No signal SAMPLE human skeletal muscle extract from BioChain PRIMARY ANTIBODY ab8991 anti beta 1D integrin from ABCAM, 1/1000 or 1/200 over night at 4?C Wash ...

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    We have just received the following information from the source of ab8991: "We would suggest to increase the protein concentration in the gel and to use more concentrated antibody for the blotting as this antibody is supplied as tissue culture supe...

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    What are the recommended dilutions on this antibody for Westerns and immunohistochemistry

    Thank you for your enquiry. For Western blotting, I would suggest starting at a dilution of 1:1000. For IHC, I would recommend titrating the antibody 1:20 to 1:200. For both applications, you will need to optimize based upon results. Please contact...

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    Can I use this antibody to test rat or mice tissues?

    We would expect this antibody to work on rat and mouse tissues, but cannot guarantee it. This epitope is conserved across species.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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