Overview

  • Product name
    Anti-Integrin beta 1d antibody [2B1]
  • Description
    Mouse monoclonal [2B1] to Integrin beta 1d
  • Host species
    Mouse
  • Specificity
    Recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: C-QENPIYKSPINNFKNPNYGRKAGL conjugated to KLH.

  • Epitope
    Cytoplasmic domain, C-terminal. Isoform of the Integrin b1 subunit present in cardiac and skeletal muscle.
  • General notes


    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.
  • Clonality
    Monoclonal
  • Clone number
    2B1
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000.
IHC-Fr 1/25 - 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. The isoform beta-1D of Integrin 1, is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • CD29 antibody
    • FNRB antibody
    • Integrin beta 1 antibody
    • ITGB 1 antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • VLAB antibody
    see all

Images

  • Western blotting of beta1D integrin showing its muscle cell specific expression pattern.

    Western blotting of beta1D integrin showing its muscle cell specific expression pattern.
  • Immunofluorescence staining of myocardium for beta1D integrin.

References

This product has been referenced in:
  • Yuan T  et al. Low-density lipoprotein receptor-related protein 6 regulates alternative pre-mRNA splicing. J Cell Mol Med 22:4653-4663 (2018). Read more (PubMed: 30070011) »
  • Lee HJ  et al. Dysregulation of nuclear receptor COUP-TFII impairs skeletal muscle development. Sci Rep 7:3136 (2017). WB, IF ; Mouse . Read more (PubMed: 28600496) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Please forward the following publication to customer, there are many markers listed and we have antibodies against these. Customer might need to make decision based on their requirements.

http://journals.tums.ac.ir/upload_files/pdf/_/20047.pdf

The cardiac cell surface markers are like; KCNA6; https://www.abcam.com/kv16-antibody-ab21089.html

https://www.abcam.com/popdc2-antibody-ab69176.html

β1- and β2-adrenergic receptors etc.

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Answer


I would recommend to use an anti-integrin beta 1d such as https://www.abcam.com/ab8992 or https://www.abcam.com/ab8991.

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Answer

Thank you for your enquiry. I am sorry it has taken a little time to obtain this protocol for you. Thank you for your patience. I hope this will be helpflu to you. Please note that this procedure would be a guideline only and may require some further optmization in your laboratory. Indirect immunofluorescence protocol for ab8991 Integrin beta 1d antibody [2B1]: 1. Cut 5µm sections of the frozen tissue with a thickness of 5 µm. 2. Allow them to dry a few minutes at room temperature. 3. Fixation: 20 min in aceton at -20°C, make sure the aceton is pre-cooled. 4. Wash 3 times for 5 min with PBS on a shaker. 5. Dissolve the primary antibody in PBS/ 0,1 % BSA according to the instructions in the datasheet. 6. Add minimally 100 µl diluted antibody to the tissue section. Put the slides in a closed box with wet tissues. It will keep the humidity high and prevents drying of the antibody solution. 7. Incubate the slides 60 minutes at 37°C. 8. Rinse the slides PBS. 9. Wash 3 times for 5 min with PBS on a shaker. Always use a different jar per antibody and wash the negative control separately. 10. Dilution the secondary FITC conjugated antibody (rabbit anti-mouse) 1:100 in PBS/ 0.1 % BSA. 11. Add minimally 100 µl diluted secondary antibody to the tissue section. Put again the slides in a closed box with wet tissues. 12. Incubate the slides 30 minutes at 37°C 13. Wash 3 times for 5 min with PBS on a shaker 14. Dehydrate the slides in an ascending ethanol series, 70%, 96% and 100%. 15. Allow the slides to dry. It is important that the tissue section is completely dry. 16. Mount slides with 0.5 µg DAPI/ml in the mounting medium (50% glycerol in 0.02 M TRIS HCl; pH7.4, containing 2% 1,4-diazobicyclo(2,2,2)-octane (DABCO)). 17. Absorb the excess of mounting medium. 18. Use nail polish to adhere the cover glass to the slides. 19. Observe in fluorescence microscope. If you have any further questions, please do not hesitate to contact us.

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Answer

We have just received the following information from the source of ab8991: "We would suggest to increase the protein concentration in the gel and to use more concentrated antibody for the blotting as this antibody is supplied as tissue culture supernatant." I apologise for the delay in replying to you, please be assured that if you still have a problem we will send you a replacement or refund if you purchased the antibody in the last 90 days.

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Question
Answer

Thank you for your enquiry. For Western blotting, I would suggest starting at a dilution of 1:1000. For IHC, I would recommend titrating the antibody 1:20 to 1:200. For both applications, you will need to optimize based upon results. Please contact us again if you have any additional questions.

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Question
Answer

We would expect this antibody to work on rat and mouse tissues, but cannot guarantee it. This epitope is conserved across species.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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