Overview

  • Product name

    Anti-Integrin beta 3 antibody
    See all Integrin beta 3 primary antibodies
  • Description

    Rabbit polyclonal to Integrin beta 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Fusion protein corresponding to Human Integrin beta 3 (internal sequence). (BC127666).
    Database link: P05106

  • Positive control

    • Jurkat whole cell lysate (ab7899); Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab197662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 87 kDa.
IHC-P 1/25 - 1/100.

Target

  • Function

    Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Tissue specificity

    Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
  • Involvement in disease

    Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD 61 antibody
    • CD61 antibody
    • CD61 antigen antibody
    • GP3A antibody
    • GPIIIa antibody
    • GT antibody
    • HPA 1 antibody
    • HPA 4 antibody
    • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
    • Integrin beta chain beta 3 antibody
    • Integrin beta-3 antibody
    • ITB3_HUMAN antibody
    • ITG B3 antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • NAIT antibody
    • platelet fibrinogen receptor antibody
    • Platelet fibrinogen receptor beta subunit antibody
    • Platelet fibrinogen receptor, beta subunit antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • PTP antibody
    see all

Images

  • Anti-Integrin beta 3 antibody (ab197662) at 1/450 dilution + Jurkat cell lysate at 40 µg

    Predicted band size: 87 kDa


    Exposure time: 10 seconds


    8% SDS-PAGE.

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Integrin beta 3 with ab197662 at 1/20 dilution.

References

This product has been referenced in:

  • Faralli JA  et al. Effect of avß3 Integrin Expression and Activity on Intraocular Pressure. Invest Ophthalmol Vis Sci 60:1776-1788 (2019). Read more (PubMed: 31022732) »
  • Faralli JA  et al. Absence of a secondary glucocorticoid response in C57BL/6J mice treated with topical dexamethasone. PLoS One 13:e0192665 (2018). Read more (PubMed: 29499052) »
See all 5 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197662.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up