Recombinant Anti-Integrin beta 3 antibody [EPR2342] (ab119992)


  • Product name
    Anti-Integrin beta 3 antibody [EPR2342]
    See all Integrin beta 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR2342] to Integrin beta 3
  • Host species
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Integrin beta 3 aa 750-850. The exact sequence is proprietary.

  • Positive control
    • WB: THP-1 treated with TPA, HUVEC, Human spleen and Human plasma lysates. Flow Cyt: HUVEC cells.
  • General notes

    A trial size is available to purchase for this antibody.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab119992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
IP 1/50.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Tissue specificity
      Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • Involvement in disease
      Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all


    • All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500 dilution

      Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates with 5% NFDM/TBST
      Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates with 5% NFDM/TBST
      Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

      LnCaP, HeLa and PC-3 have low expression controls.


      1,2 lane: 10 s;

      3-8 lanes: 3 min

    • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) cell line labeling Integrin beta 3 with ab119992 at 1/20 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    • All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution

      Lane 1 : TPA treated THP1 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 87 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?


    This product has been referenced in:
    See all 11 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for contacting us.

    After checking our antibodies, ab131056 may be suitable for your purposes. The immunogen is 100% homologous with mouse and only 3 amino acids are not conserved in the 3b and 3c isoforms so it should show cross-reactivity with all three isoforms. It has been validated for use in IHC-P and WB on human and rat samples, but has not been tested on cultured cells or mouse samples.

    If you would like to test this antibody, I would be happy to offer you our 100% testing discount program. If you purchase the antibody for full price and submit an Abreview with your mouse results, I will send you a discount code that can be used to receive a free antibody on a future purchase. For more information about this program, please visit:

    If you are interested in testing this antibody, please let me know and I will be happy to send you the discount code.

    I am also in the process of checking whether ab119992 would be suitable, and I will let you know as soon as I have any further information from the lab.

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    Based on sequence analysis, the ab119992 is expected to detect only the isoform 3A of Integrin beta 3.
    I hope this is helpful. Please let me know if you have any further questions.

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