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Cardiovascular Angiogenesis Adhesion / ECM Integrins
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RecombinantRabMAb

Recombinant Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (17)

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Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
  • Flow Cytometry - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
  • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
  • Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2342] to Integrin beta 3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Integrin beta 3 antibody [EPR2342]
    See all Integrin beta 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR2342] to Integrin beta 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87MG,HEL,C2C12,THP-1 treated with TPA, HUVEC, Human spleen and Human plasma lysates. Flow Cyt: HUVEC cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2342
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Vascular remodelling
    • Integrins

Associated products

  • Alternative Versions

    • Anti-Integrin beta 3 antibody [EPR2342] - BSA and Azide free (ab239991)
    • Anti-Integrin beta 3 antibody [EPR2342] - Low endotoxin, Azide free (ab246708)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
Flow Cyt
1/20.
Notes
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
Flow Cyt
1/20.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Tissue specificity

      Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • Involvement in disease

      Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P05106 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3690 Human
      • Entrez Gene: 16416 Mouse
      • Entrez Gene: 29302 Rat
      • Omim: 173470 Human
      • SwissProt: P05106 Human
      • SwissProt: O54890 Mouse
      • Unigene: 218040 Human
      • Unigene: 87150 Mouse
      • Unigene: 229225 Rat
      see all
    • Alternative names

      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • platelet fibrinogen receptor antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
      Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
      All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500 dilution

      Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates with 5% NFDM/TBST
      Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates with 5% NFDM/TBST
      Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?



      LnCaP, HeLa and PC-3 are low expression controls.

      Exposure:

      1,2 lane: 10 s;

      3-8 lanes: 3 min

    • Flow Cytometry - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
      Flow Cytometry - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HUVEC (Human umbilical vein endothelial cell) cell line labeling Integrin beta 3 with ab119992 at 1/20 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    • Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
      Western blot - Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
      All lanes : Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/1000 dilution

      Lane 1 : TPA treated THP1 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 87 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Anti-Integrin beta 3 antibody [EPR2342] (ab119992)
      Anti-Integrin beta 3 antibody [EPR2342] (ab119992)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (17)

    Publishing research using ab119992? Please let us know so that we can cite the reference in this datasheet.

    ab119992 has been referenced in 17 publications.

    • Larios J  et al. ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes. J Cell Biol 219:N/A (2020). PubMed: 32049272
    • Chen J  et al. Remarkable Boron Delivery Of iRGD-Modified Polymeric Nanoparticles For Boron Neutron Capture Therapy. Int J Nanomedicine 14:8161-8177 (2019). PubMed: 31632025
    • Guo G  et al. Microarray analyses of lncRNAs and mRNAs expression profiling associated with diabetic peripheral neuropathy in rats. J Cell Biochem N/A:N/A (2019). PubMed: 31025414
    • Li Y  et al. Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer. Elife 8:N/A (2019). PubMed: 31012847
    • He F  et al. Reversal of EGFR inhibitors' resistance by co-delivering EGFR and integrin avß3 inhibitors with nanoparticles in non-small cell lung cancer. Biosci Rep 39:N/A (2019). PubMed: 31316001
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I am searching for an antibody to Integrin beta 3 that will recognize all isoforms of beta 3 (does not bind in the region from aa768-788) and will cross-react with mouse and human for use in ICC.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 21 2012

    Answer

    Thank you for contacting us.

    After checking our antibodies, ab131056 may be suitable for your purposes. The immunogen is 100% homologous with mouse and only 3 amino acids are not conserved in the 3b and 3c isoforms so it should show cross-reactivity with all three isoforms. It has been validated for use in IHC-P and WB on human and rat samples, but has not been tested on cultured cells or mouse samples.

    If you would like to test this antibody, I would be happy to offer you our 100% testing discount program. If you purchase the antibody for full price and submit an Abreview with your mouse results, I will send you a discount code that can be used to receive a free antibody on a future purchase. For more information about this program, please visit:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998

    If you are interested in testing this antibody, please let me know and I will be happy to send you the discount code.

    I am also in the process of checking whether ab119992 would be suitable, and I will let you know as soon as I have any further information from the lab.

    Read More

    Abcam Scientific Support

    Answered on Jun 21 2012

    Question

    Will this antibody detect all three forms (3A, 3B and 3C) of integrin beta 3?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 20 2012

    Answer

    Based on sequence analysis, the ab119992 is expected to detect only the isoform 3A of Integrin beta 3.
    I hope this is helpful. Please let me know if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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