RecombinantRabMAb

Recombinant Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Immunoprecipitation - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
  • Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [ERP17507] to Integrin beta 3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Integrin beta 3 antibody [ERP17507]
    See all Integrin beta 3 primary antibodies

  • Description

    Rabbit monoclonal [ERP17507] to Integrin beta 3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29, HEK-293, HUVEC, HeLa and HEL 92.1.7 whole cell lysates; Human spleen lysate; Human plasma. IHC-P: Human spleen, osteosarcomas and thyroid cancer tissues. IP: HUVEC whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 130, 97 kDa (predicted molecular weight: 87 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Notes
WB
1/1000. Detects a band of approximately 130, 97 kDa (predicted molecular weight: 87 kDa).
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.

Target

  • Function

    Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Tissue specificity

    Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.

  • Involvement in disease

    Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.

  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • platelet fibrinogen receptor antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Positive staining on platelets (PMID: 25356112) of Human spleen is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      All lanes : Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1/500 dilution

      Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates with 5% NFDM/TBST
      Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
      Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates with 5% NFDM/TBST
      Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST
      Lane 9 : Mouse brain lysates with 5% NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 87 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      LnCaP, HeLa and PC-3 are low expression controls.

    • Immunoprecipitation - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Immunoprecipitation - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

      Purified ab179473 at 1/30 dilution (2µg) immunoprecipitating Integrin beta 3 in HUVEC whole cell lysate.
      Lane 1 (input): HUVEC (human umbilical vein endothelial cell) whole cell lysate 10 µg
      Lane 2 (+): ab179473 + HUVEC whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab179473 in HUVEC whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 110 kDa

    • Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1000 cells + HEL 92.1.7 (Human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 87 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with the description in the literature (PMID: 1710517).

    • Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Western blot - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Anti-Integrin beta 3 antibody [ERP17507] (ab179473) at 1/5000 dilution + Human plasma at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 87 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with the description in the literature (PMID: 1710517).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

      Immunohistochemical analysis of paraffin-embedded Human osteosarcomas tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Membrane staining on cells of Human osteosarcomas is observed; several multinucleated osteoclasts are positively stained (PMID: 15692727; PMID: 25534583).

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

      Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Integrin beta 3 with ab179473 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Membrane or cytoplasmic staining on tumor cells of Human thyroid cancer is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Integrin beta 3 antibody [ERP17507] (ab179473)
      Anti-Integrin beta 3 antibody [ERP17507] (ab179473)

    References (1)

    Publishing research using ab179473? Please let us know so that we can cite the reference in this datasheet.

    ab179473 has been referenced in 1 publication.

    • Kazmierczak PM  et al. Integrin-targeted quantitative optoacoustic imaging with MRI correlation for monitoring a BRAF/MEK inhibitor combination therapy in a murine model of human melanoma. PLoS One 13:e0204930 (2018). PubMed: 30281669

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