Overview

  • Product name
    Anti-Integrin beta 3 antibody [MHF4]
    See all Integrin beta 3 primary antibodies
  • Description
    Mouse monoclonal [MHF4] to Integrin beta 3
  • Host species
    Mouse
  • Specificity
    MHF4 may be used to identify osteoclasts in tissue sections and bone cell suspensions.
  • Tested applications
    Suitable for: IP, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Cell preparation: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.

  • General notes
    A heterodimeric molecule with a characteristic shift in apparent molecular mass on reduction (137/78 kDa nonreduced; 118/100 kDa reduced).

Properties

Applications

Our Abpromise guarantee covers the use of ab20146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Flow Cyt
IHC-Fr
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    Note: samples should be acetone fixed.

    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Tissue specificity
      Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • Involvement in disease
      Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    References

    This product has been referenced in:
    • Ponceau A  et al. aII-spectrin regulates invadosome stability and extracellular matrix degradation. PLoS One 10:e0120781 (2015). Read more (PubMed: 25830635) »
    • Helfrich MH  et al. Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3. J Bone Miner Res 7:345-51 (1992). Read more (PubMed: 1585837) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Toutes nos garanties d'applications et espèces pour chaque produit sont spécifiées dans la fiche technique, et sont mises à jour régulièrement. Comme indiqué sur la fiche technique (www.abcam.com/ab20146), l'anti-Integrin beta 3 référence ab20146 a été testé en cytométrie en flux, immunoprécipitation et immunohistochimie sur coupes cryo-congelées. Les espèces testées sont l'homme et le rat. Si vous désirez utiliser cet anticorps dans une nouvelle application ou espèce, nous pouvons vous proposer une offre de test en échange de vos résultats sous forme d'Abreview. 1) Nous vous attribuons un code promotionnel 2) Vous achetez l'anticorps à tester 3) Vous testez l'anticorps 4) Que le résultat soit positif ou non, vous soumettez une Abreview (https://www.abcam.com/abreviews) et le code sera activé 5) Le code vous permet de commander gratuitement n'importe quel anticorps primaire du catalogue Abcam. J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

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