Key features and details
- Rabbit monoclonal [SP324] to Integrin beta 3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Integrin beta 3 antibody [SP324]
See all Integrin beta 3 primary antibodies
DescriptionRabbit monoclonal [SP324] to Integrin beta 3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Synthetic peptide corresponding to Human Integrin beta 3 (internal sequence).
Database link: P05106
- WB: HUVEC cell lysate. IHC-P: Human bone marrow and spleen tissues.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab227702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Predicted molecular weight: 87 kDa.
Primary antibody incubation for 1 hour at room temperature.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.
Primary antibody incubation for 10 minutes at room temperature.
FunctionIntegrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Tissue specificityIsoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Involvement in diseaseDefects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
modificationsPhosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
- Information by UniProt
- BDPLT16 antibody
- BDPLT2 antibody
- CD 61 antibody
All lanes : Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/500 dilution
Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates
Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 9 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
LnCaP, HeLa and PC-3 are low expression controls. This product doesn’t react with Ms
Formalin-fixed, paraffin-embedded human spleen tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.
Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/100 dilution + HUVEC (human umbilical vein endothelial cell line) cell lysate
Predicted band size: 87 kDa
Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227702 has not yet been referenced specifically in any publications.