Overview

  • Product name

    Anti-Integrin beta 3 antibody [SP324]
    See all Integrin beta 3 primary antibodies
  • Description

    Rabbit monoclonal [SP324] to Integrin beta 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Integrin beta 3 (internal sequence).
    Database link: P05106

  • Positive control

    • WB: HUVEC cell lysate. IHC-P: Human bone marrow and spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 87 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Tissue specificity

    Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
  • Involvement in disease

    Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD 61 antibody
    • CD61 antibody
    • CD61 antigen antibody
    • GP3A antibody
    • GPIIIa antibody
    • GT antibody
    • HPA 1 antibody
    • HPA 4 antibody
    • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
    • Integrin beta chain beta 3 antibody
    • Integrin beta-3 antibody
    • ITB3_HUMAN antibody
    • ITG B3 antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • NAIT antibody
    • platelet fibrinogen receptor antibody
    • Platelet fibrinogen receptor beta subunit antibody
    • Platelet fibrinogen receptor, beta subunit antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • PTP antibody
    see all

Images

  • All lanes : Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/500 dilution

    Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
    Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates
    Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates
    Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 9 : Mouse brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    LnCaP, HeLa and PC-3 are low expression controls. This product doesn’t react with Ms

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Integrin beta 3 antibody [SP324] (ab227702) at 1/100 dilution + HUVEC (human umbilical vein endothelial cell line) cell lysate

    Predicted band size: 87 kDa

  • Formalin-fixed, paraffin-embedded human bone marrow tissue stained for Integrin beta 3 using ab227702 at 1/100 dilution in immunohistochemical analysis.

References

ab227702 has not yet been referenced specifically in any publications.

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