Anti-Integrin beta 3 antibody [VI-PL2] (ab110131)


  • Product name
    Anti-Integrin beta 3 antibody [VI-PL2]
    See all Integrin beta 3 primary antibodies
  • Description
    Mouse monoclonal [VI-PL2] to Integrin beta 3
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length CD61.

  • Positive control
    • Human placenta and spleen tissues; Normal Human peripheral blood platelets.



Our Abpromise guarantee covers the use of ab110131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 87 kDa.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 0.125µg for 105-8 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Tissue specificity
    Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
  • Involvement in disease
    Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD 61 antibody
    • CD61 antibody
    • CD61 antigen antibody
    • GP3A antibody
    • GPIIIa antibody
    • GT antibody
    • HPA 1 antibody
    • HPA 4 antibody
    • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
    • Integrin beta chain beta 3 antibody
    • Integrin beta-3 antibody
    • ITB3_HUMAN antibody
    • ITG B3 antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • NAIT antibody
    • Platelet fibrinogen receptor beta subunit antibody
    • Platelet fibrinogen receptor, beta subunit antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • PTP antibody
    see all


  • Formalin fixed, paraffin embedded Human placenta tissue labelled with ab110131 at 20 µg/ml.
  • Formalin fixed, paraffin embedded Human spleen tissue labelled with ab110131 at 20 µg/ml.
  • Staining of normal Human peripheral blood platelets with 0.06 µg ab110131 followed by PE Donkey Anti-Mouse IgG (H+L). Samples were then co-stained with FITC anti-Human CD42b to label platelets.


This product has been referenced in:
  • Wei X  et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol 216:1455-1471 (2017). Read more (PubMed: 28408404) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. I have attempted to contact you by phone but thought I might communicate this information by email as well. I have reviewed of our catalog for mouse monoclonalAnti-Integrin beta 3 antibodies for you. I have also spoken w...

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The link to all our Easylink conjugation kits is:

The smaller sized kit we talked about is ab102892:µg-hrp-ab1028...

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Thank you very much for sending this information. I am sorry that these antibodies did not work as expected. I have issued the credits to Cedarlane, and I will contact them to pass these credits on to you. Please let me know if you have...

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Thanks so much for forwarding the images. I am sorry to see that the results were so poor. Could you also send the general protocol that was used? As mentioned previously, I can send replacements or issue credit or refunds for the integrin beta...

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Thanks for your reply and for clarifying that, and I look forward to receiving any data or images that you can provide. Please let me know if you need anything else from me.

Thank you very much for your replies and for sending the information. I am sorry to hear that none of these antibodies gave the expected results. Just to clarify, there were no bands in Western blot even with the human protein samples? We do guarantee ...

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Thank you for your inquiry and I'm sorry to hear that your customer is having trouble with these products. As for ab66253, we haven't tested this antibody in endogenous samples, so we are unsure how it will work with brain tissue.  However, we have...

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