Overview

  • Product name
    Anti-Integrin beta 4 antibody [346-11A]
    See all Integrin beta 4 primary antibodies
  • Description
    Rat monoclonal [346-11A] to Integrin beta 4
  • Host species
    Rat
  • Specificity
    ab25254 recognises Integrin ß4 chain (CD104), Mr 200 kDa.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    100mM borate buffered saline, pH 8.0.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    346-11A
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration. PubMed: 20094062
IHC-Fr Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.

Target

  • Function
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Images

  • This image shows a acetone fixed frozen section of BALB/c mouse intestine. The basal layer stains intensely (arrow).
  • Immunohistochemistry (Frozen sections) analysis of BALB/c mouse frozen intestine. Right: Integrin beta 4 labeled with ab25254 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Left: Isotype Control, Rat IgG2a-UNLB followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Blue - DAPI

  • ab25254 staining mouse small intestine tissue sections by IHC-Fr.  Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C.  A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary.

    See Abreview

  • ab25254 staining Intergrin beta 4 in mouse colon cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100) for 30 minutes at 20°C. An undiluted Alexa Fluor® 647-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Gating Strategy: Exclude small cells/debris.

    See Abreview

References

This product has been referenced in:
  • Carroll TD  et al. Interkinetic nuclear migration and basal tethering facilitates post-mitotic daughter separation in intestinal organoids. J Cell Sci 130:3862-3877 (2017). Read more (PubMed: 28982714) »
  • Larkins CE  et al. Spatiotemporal dynamics of androgen signaling underlie sexual differentiation and congenital malformations of the urethra and vagina. Proc Natl Acad Sci U S A 113:E7510-E7517 (2016). Read more (PubMed: 27821748) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Flow Cytometry
Permeabilization
No
Sample
Mouse Cell (mouse colon)
Specification
mouse colon
Gating Strategy
exclude small cells/debris
Preparation
Cell harvesting/tissue preparation method: crypt isolation
Sample buffer: dmem

Abcam user community

Verified customer

Submitted Oct 03 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Cell (colon epithelial)
Specification
colon epithelial
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2014

Answer

DISCOUNT CODES:

ab25254 - ***
ab110167 - ***

Expiration date: ***

I am very pleased to hear you would like to accept our offer and test ab25254 in flow cytometry, and ab110167 on mouse samples. To redeem this offer, please submit Abreviews for flow cytometry on mouse samples and include the appropriate code in the “Additional Comments” section so we know the Abreviews are for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small intestine)
Specification
Small intestine
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up