Anti-Integrin beta 4 antibody [439-9B] (ab110167)

Rat monoclonal Integrin beta 4 antibody [439-9B]. Validated in WB, IHC, Flow Cyt and tested in Human. Cited in 2 publication(s). Independently reviewed in 1 review(s).

Reviews (1)Q&A (1)References (4)

Overview

  • Product name

    Anti-Integrin beta 4 antibody [439-9B]
    See all Integrin beta 4 primary antibodies

  • Description

    Rat monoclonal [439-9B] to Integrin beta 4

  • Host species

    Rat

  • Specificity

    Recognizes human Integrin beta-4, a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. It is used as a surface staining antibody.

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Other Immunogen Type corresponding to Human Integrin beta 4.
    Database link: P16144

  • Positive control

    • Human Pancreas and Human Placenta tissues, A549 cell line

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    439-9B

  • Isotype

    IgG2b

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 105-8 cells.

in 100 µl

 

 

 

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.

  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

  • Involvement in disease

    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.

  • Domain

    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

      ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Pancreas(ab29816). Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

      ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Placenta. Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

    • Flow Cytometry - Anti-Integrin beta 4 antibody [439-9B] (ab110167)
      Flow Cytometry - Anti-Integrin beta 4 antibody [439-9B] (ab110167)

      Staining of the A549 cell line with 0.5 ug of Rat IgG2b Isotype Control (ab98984) (blue histogram) or 0.5 ug of ab110167 (purple histogram) followed by Biotin Anti-Rat IgG and Sav-PE. Total viable cells were used for analysis.

    References

    This product has been referenced in:

    • Wang M  et al. Integrin a6ß4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions. Mol Biol Cell 30:838-850 (2019). Read more (PubMed: 30865564) »
    • Brown CW  et al. The a6ß4 integrin promotes resistance to ferroptosis. J Cell Biol 216:4287-4297 (2017). Read more (PubMed: 28972104) »
    See all 4 Publications for this product

    Publishing research using ab110167? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 4 antibody [439-9B]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hepatocellular carcinoma)
    Permeabilization
    Yes - Triton X-100
    Specification
    Hepatocellular carcinoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 22 2018

    Question

    Please send two testing discount codes for two integrin beta 4 antibodies for flow cytometry of mouse samples.

    Read More
    Answer

    DISCOUNT CODES:

    ab25254 - ***
    ab110167 - ***

    Expiration date: ***

    I am very pleased to hear you would like to accept our offer and test ab25254 in flow cytometry, and ab110167 on mouse samples. To redeem this offer, please submit Abreviews for flow cytometry on mouse samples and include the appropriate code in the “Additional Comments” section so we know the Abreviews are for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    We publish positive and negative Abreviews on our datasheets so please submit the results of your tests regardless of the outcome. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com