Key features and details
- Mouse monoclonal [450-9D] to Integrin beta 4 (FITC)
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-Integrin beta 4 antibody [450-9D] (FITC)
See all Integrin beta 4 primary antibodies
DescriptionMouse monoclonal [450-9D] to Integrin beta 4 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificityab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Full length native protein (purified) (Human): Purified a6b4 integrin from A431 cells
Epitopethe epitope is in the extracellular region of Integrin beta 4
- Human tonsil
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab22486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.
DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
- Information by UniProt
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
ab22486 has been referenced in 3 publications.
- Gamage TKJB et al. Human trophoblasts are primarily distinguished from somatic cells by differences in the pattern rather than the degree of global CpG methylation. Biol Open 7:N/A (2018). PubMed: 30026266
- James JL et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). PubMed: 26248480
- DaSilva-Arnold S et al. Differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition. Placenta 36:1412-8 (2015). PubMed: 26545962