• Product name

    Anti-Integrin beta 4 antibody [450-9D] (FITC)
    See all Integrin beta 4 primary antibodies
  • Description

    Mouse monoclonal [450-9D] to Integrin beta 4 (FITC)
  • Host species

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.
  • Tested applications

    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human): Purified a6b4 integrin from A431 cells

  • Epitope

    the epitope is in the extracellular region of Integrin beta 4
  • Positive control

    • Human tonsil


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality

  • Clone number

  • Myeloma

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab22486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/10.

Use 10ul of the suggested working dilution to label 106 cells in 100ul.




ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function

    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease

    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain

    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all


  • Immunohistochemistry (Frozen sections) analysis of human tonsil tissue section labeling Integrin beta 4 with ab22486. 


This product has been referenced in:

  • James JL  et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). Read more (PubMed: 26248480) »
  • DaSilva-Arnold S  et al. Differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition. Placenta 36:1412-8 (2015). Read more (PubMed: 26545962) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Further to previous correspondence I have been in touch with the source of this antibody. I am concerned that this antibody has in fact NOT been tested and shown to work by western blotting. It is FITC labeled and therefore my concern was that the fluorophore label was occluding the capacity for the secondary to recognise the primary antibody. Given that it is yet to be tested I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

Read More


Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I appreciate the controls that you have in place and that you are detecting Integrin beta 4 using an alternatively sourced antibody. Please can you detail the antibody dilutions that you have applied the antibody at and whether the alternative antibody was applied in parallel to control for variation in your experimental procedure. I am in touch with the lab to determine the conditions employed as my concern is that given this antibody is FITC conjugated and may occlude the binding of a secondary antibody. I look forward to hearing from you.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up