Recombinant
RabMAb

Recombinant Anti-Integrin beta 4 antibody [EPR8559] (ab133682)

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Overview

  • Product name

    Anti-Integrin beta 4 antibody [EPR8559]
    See all Integrin beta 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR8559] to Integrin beta 4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Integrin beta 4 aa 1750-1850 (C terminal). The exact sequence is proprietary.

  • Positive control

    • BxPC-3 and SW480 cell lysates; BxPC-3 cells

  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 9.70 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8559

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 202 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.

    • Tissue specificity

      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.

    • Involvement in disease

      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.

    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.

    • Domain

      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 104 antibody
        • CD104 antibody
        • CD104 antigen antibody
        • gp150 antibody
        • Integrin beta 4 subunit antibody
        • Integrin beta-4 antibody
        • ITB4_HUMAN antibody
        • ITG B4 antibody
        • ITGB 4 antibody
        • Itgb4 antibody
        see all

      Images

      • Western blot - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        Western blot - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        All lanes : Anti-Integrin beta 4 antibody [EPR8559] (ab133682) at 1/1000 dilution

        Lane 1 : BxPC-3 cell lysate
        Lane 2 : SW480 cell lysate

        Lysates/proteins at 10 µg/ml per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 202 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)This image is courtesy of an Abreview submitted by Qingsu Cheng

        ab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution.
      • Other - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        Other - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Jin SH  et al. M. leprae interacts with the human epidermal keratinocytes, neonatal (HEKn) via the binding of laminin-5 with a-dystroglycan, integrin-ß1, or -ß4. PLoS Negl Trop Dis 13:e0007339 (2019). Read more (PubMed: 31233498) »
      • Lü Y  et al. Berberine regulates the microRNA-21-ITG?4-PDCD4 axis and inhibits colon cancer viability. Oncol Lett 15:5971-5976 (2018). WB . Read more (PubMed: 29564000) »
      See all 5 Publications for this product

      Publishing research using ab133682? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 4 antibody [EPR8559]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
      Sample
      Human Cell (Breast epithelial)
      Specification
      Breast epithelial
      Permeabilization
      Yes - Triton X-100
      Fixative
      Paraformaldehyde
      Read More

      Dr. Qingsu Cheng

      Verified customer

      Submitted May 29 2014

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