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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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RecombinantRabMAb

Recombinant Anti-Integrin beta 4 antibody [EPR8559] (ab133682)

  • Datasheet
Reviews (1) Submit a question References (8)

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Western blot - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
  • OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
  • Anti-Integrin beta 4 antibody [EPR8559] (ab133682)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8559] to Integrin beta 4
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Carrier Free

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Overview

  • Product name

    Anti-Integrin beta 4 antibody [EPR8559]
    See all Integrin beta 4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8559] to Integrin beta 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Integrin beta 4 aa 1750-1850 (C terminal). The exact sequence is proprietary.

  • Positive control

    • BxPC-3 and SW480 cell lysates; BxPC-3 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 9.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8559
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta

Associated products

  • Alternative Versions

    • Anti-Integrin beta 4 antibody [EPR8559] - BSA and Azide free (ab240078)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Integrin beta 4 protein (His tag) (ab229512)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 202 kDa.
ICC/IF (1)
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 202 kDa.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease

    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain

    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P16144 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3691 Human
    • Omim: 147557 Human
    • SwissProt: P16144 Human
    • Unigene: 632226 Human
    • Alternative names

      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      Western blot - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      All lanes : Anti-Integrin beta 4 antibody [EPR8559] (ab133682) at 1/1000 dilution

      Lane 1 : BxPC-3 cell lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg/ml per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 202 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)This image is courtesy of an Abreview submitted by Qingsu Cheng

      ab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution.
    • OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Integrin beta 4 antibody [EPR8559] (ab133682)
      Anti-Integrin beta 4 antibody [EPR8559] (ab133682)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (8)

    Publishing research using ab133682? Please let us know so that we can cite the reference in this datasheet.

    ab133682 has been referenced in 8 publications.

    • Gao Q  et al. Functional Passenger-Strand miRNAs in Exosomes Derived from Human Colon Cancer Cells and Their Heterogeneous Paracrine Effects. Int J Biol Sci 16:1044-1058 (2020). PubMed: 32140072
    • Cheng Q  et al. Overexpression of CD36 in mammary fibroblasts suppresses colony growth in breast cancer cell lines. Biochem Biophys Res Commun 526:41-47 (2020). PubMed: 32192771
    • Jiang K  et al. Exosome-derived ENO1 regulates integrin a6ß4 expression and promotes hepatocellular carcinoma growth and metastasis. Cell Death Dis 11:972 (2020). PubMed: 33184263
    • Jin SH  et al. M. leprae interacts with the human epidermal keratinocytes, neonatal (HEKn) via the binding of laminin-5 with a-dystroglycan, integrin-ß1, or -ß4. PLoS Negl Trop Dis 13:e0007339 (2019). PubMed: 31233498
    • Lü Y  et al. Berberine regulates the microRNA-21-ITG?4-PDCD4 axis and inhibits colon cancer viability. Oncol Lett 15:5971-5976 (2018). WB . PubMed: 29564000
    • Colburn ZT & Jones JCR Complexes of a6ß4 integrin and vimentin act as signaling hubs to regulate epithelial cell migration. J Cell Sci 131:N/A (2018). PubMed: 29976561
    • Domke LM & Franke WW The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system. Cell Tissue Res N/A:N/A (2018). PubMed: 30591979
    • Lee JY  et al. Depletion of CTCF in Breast Cancer Cells Selectively Induces Cancer Cell Death via p53. J Cancer 8:2124-2131 (2017). PubMed: 28819414

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 4 antibody [EPR8559]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (Breast epithelial)
    Specification
    Breast epithelial
    Permeabilization
    Yes - Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    DR. Qingsu Cheng

    Verified customer

    Submitted May 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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