Product nameAnti-Integrin beta 4 antibody [EPR8559]
See all Integrin beta 4 primary antibodies
DescriptionRabbit monoclonal [EPR8559] to Integrin beta 4
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Integrin beta 4 aa 1750-1850 (C terminal). The exact sequence is proprietary.
- BxPC-3 and SW480 cell lysates; BxPC-3 cells
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 202 kDa.|
|ICC/IF||1/250 - 1/500.|
FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain.
DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
- Information by UniProt
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
All lanes : Anti-Integrin beta 4 antibody [EPR8559] (ab133682) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 10 µg/ml per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 202 kDa
ab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Jin SH et al. M. leprae interacts with the human epidermal keratinocytes, neonatal (HEKn) via the binding of laminin-5 with a-dystroglycan, integrin-ß1, or -ß4. PLoS Negl Trop Dis 13:e0007339 (2019). Read more (PubMed: 31233498) »
- Lü Y et al. Berberine regulates the microRNA-21-ITG?4-PDCD4 axis and inhibits colon cancer viability. Oncol Lett 15:5971-5976 (2018). WB . Read more (PubMed: 29564000) »