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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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Anti-Integrin beta 4 antibody [M126] (ab29042)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (17)

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Flow Cytometry - Anti-Integrin beta 4 antibody [M126] (ab29042)
  • Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)

Key features and details

  • Mouse monoclonal [M126] to Integrin beta 4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
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Protein
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Recombinant Human Integrin beta 4 protein (His tag) (ab229512)

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Overview

  • Product name

    Anti-Integrin beta 4 antibody [M126]
    See all Integrin beta 4 primary antibodies
  • Description

    Mouse monoclonal [M126] to Integrin beta 4
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.

  • Positive control

    • A431 cell lysate. 804G cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    protein A chromatography.
  • Clonality

    Monoclonal
  • Clone number

    M126
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Integrin beta 4 protein (His tag) (ab229512)
  • Related Products

    • Normal Goat Serum (ab7481)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab29042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
WB (2)
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease

    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain

    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P16144 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3691 Human
    • Omim: 147557 Human
    • SwissProt: P16144 Human
    • Unigene: 632226 Human
    • Alternative names

      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Images

    • Flow Cytometry - Anti-Integrin beta 4 antibody [M126] (ab29042)
      Flow Cytometry - Anti-Integrin beta 4 antibody [M126] (ab29042)

      Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)
      Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)
      All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)

      Lane 1 : A431 (25 µg/lane) serum starved overnight
      Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min

      Predicted band size: 202 kDa

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (17)

    Publishing research using ab29042? Please let us know so that we can cite the reference in this datasheet.

    ab29042 has been referenced in 17 publications.

    • Ma B  et al. Reciprocal regulation of integrin ß4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits. J Exp Clin Cancer Res 38:23 (2019). PubMed: 30658712
    • Yu PF  et al. Dose dependency PM2.5 aggravated airway inflammation in asthmatic mice via down-regulating expression of ITGB4. Eur Rev Med Pharmacol Sci 23:1688-1697 (2019). PubMed: 30840294
    • Elaimy AL  et al. Real-time imaging of integrin ß4 dynamics using a reporter cell line generated by Crispr/Cas9 genome editing. J Cell Sci 132:N/A (2019). PubMed: 31262785
    • Evans R  et al. Integrin-Mediated Macrophage Adhesion Promotes Lymphovascular Dissemination in Breast Cancer. Cell Rep 27:1967-1978.e4 (2019). PubMed: 31091437
    • Kasendra M  et al. Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids. Sci Rep 8:2871 (2018). PubMed: 29440725
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin beta 4 antibody [M126]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Rabbit cornea. Limbal area.)
    Permeabilization
    No
    Specification
    Rabbit cornea. Limbal area.
    Blocking step
    5% BSA + 10% FBS in PBT (PBS + 0,1% triton) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Dr. Noelia Andollo

    Verified customer

    Submitted Jul 20 2015

    Western blot abreview for Anti-Integrin beta 4 antibody [M126]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (Glioma cells)
    Specification
    Glioma cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 27 2013

    Western blot abreview for Anti-Integrin beta 4 antibody [M126]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7)
    Loading amount
    100000 cells
    Specification
    MCF7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 08 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin beta 4 antibody [M126]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (EpiSCs)
    Specification
    EpiSCs
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 0.1%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 14 2011

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