Overview

  • Product name

    Anti-Integrin beta 4 antibody [UMA9]
    See all Integrin beta 4 primary antibodies
  • Description

    Mouse monoclonal [UMA9] to Integrin beta 4
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Integrin beta 4. Human squamous cell carcinoma (UM-SCC1).
    Database link: P16144

  • Positive control

    • A431 cells, squamous cell carcinoma, colon or placenta tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    UMA9
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 202 kDa.
IP Use at 1-2 µg/mg of lysate.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µl for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease

    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain

    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

References

ab197772 has not yet been referenced specifically in any publications.

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