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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)

  • Datasheet
  • SDS
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Western blot - Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)

    Key features and details

    • Rabbit polyclonal to Integrin beta 4 (phospho Y1494)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
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    Recombinant Human Integrin beta 4 protein (His tag) (ab229512)

    View more associated products

    Overview

    • Product name

      Anti-Integrin beta 4 (phospho Y1494) antibody
      See all Integrin beta 4 primary antibodies
    • Description

      Rabbit polyclonal to Integrin beta 4 (phospho Y1494)
    • Host species

      Rabbit
    • Specificity

      ab29043 recognises all three isoforms of Integrin beta 4 phosphorilated at tyrosine 1494 (beta 4A, beta 4B and beta 4C).
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic phospho peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human integrin beta 4.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab29043 was cross adsorbed to phospho tyrosine coupled to agarose prior to immunogen affinity purification (without carrier protein).
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Beta

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Integrin beta 4 protein (His tag) (ab229512)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab29043 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/2000. Predicted molecular weight: 202 kDa.

    Block the membrane in 5% BSA (fraction V).

    Notes
    WB
    1/2000. Predicted molecular weight: 202 kDa.

    Block the membrane in 5% BSA (fraction V).

    Target

    • Function

      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • Tissue specificity

      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • Involvement in disease

      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • Domain

      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P16144 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3691 Human
      • Omim: 147557 Human
      • SwissProt: P16144 Human
      • Unigene: 632226 Human
      • Alternative names

        • CD 104 antibody
        • CD104 antibody
        • CD104 antigen antibody
        • gp150 antibody
        • Integrin beta 4 subunit antibody
        • Integrin beta-4 antibody
        • ITB4_HUMAN antibody
        • ITG B4 antibody
        • ITGB 4 antibody
        • Itgb4 antibody
        see all

      Images

      • Western blot - Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
        Western blot - Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
        All lanes : Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)

        Lane 1 : A431 (25 µg/lane) serum starved overnight
        Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with absence of peptide
        Lane 3 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1494) peptide
        Lane 4 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1526) peptide

        Predicted band size: 202 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab29043? Please let us know so that we can cite the reference in this datasheet.

      ab29043 has been referenced in 1 publication.

      • Liu SY  et al. A small molecule induces integrin ß4 nuclear translocation and apoptosis selectively in cancer cells with high expression of integrin ß4. Oncotarget 7:16282-96 (2016). PubMed: 26918348

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