Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
Key features and details
- Rabbit polyclonal to Integrin beta 4 (phospho Y1494)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin beta 4 (phospho Y1494) antibody
See all Integrin beta 4 primary antibodies -
Description
Rabbit polyclonal to Integrin beta 4 (phospho Y1494) -
Host species
Rabbit -
Specificity
ab29043 recognises all three isoforms of Integrin beta 4 phosphorilated at tyrosine 1494 (beta 4A, beta 4B and beta 4C). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human integrin beta 4.
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General notes
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Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab29043 was cross adsorbed to phospho tyrosine coupled to agarose prior to immunogen affinity purification (without carrier protein). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab29043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 202 kDa.
Block the membrane in 5% BSA (fraction V). |
| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 202 kDa. Block the membrane in 5% BSA (fraction V). |
Target
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Function
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
Involvement in disease
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
Domain
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3691 Human
- Omim: 147557 Human
- SwissProt: P16144 Human
- Unigene: 632226 Human
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Alternative names
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
Images
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Western blot - Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)All lanes : Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with absence of peptide
Lane 3 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1494) peptide
Lane 4 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1526) peptide
Predicted band size: 202 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab29043 has been referenced in 1 publication.
- Liu SY et al. A small molecule induces integrin ß4 nuclear translocation and apoptosis selectively in cancer cells with high expression of integrin ß4. Oncotarget 7:16282-96 (2016). PubMed: 26918348