Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Integrin beta 4 (phospho Y1494) antibody
See all Integrin beta 4 primary antibodies -
DescriptionRabbit polyclonal to Integrin beta 4 (phospho Y1494)
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Host speciesRabbit
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Specificityab29043 recognises all three isoforms of Integrin beta 4 phosphorilated at tyrosine 1494 (beta 4A, beta 4B and beta 4C).
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic phospho peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human integrin beta 4.
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Positive control
- A431 cells treated with pervanadate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab29043 was cross adsorbed to phospho tyrosine coupled to agarose prior to immunogen affinity purification (without carrier protein).
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab29043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| ELISA |
Application notes
ELISA: 1/4000.
WB: 1/2000 Block the membrane in 5% BSA (fraction V). Predicted molecular weight: 202 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 Block the membrane in 5% BSA (fraction V). Predicted molecular weight: 202 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
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Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
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Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 3691 Human
- Entrez Gene: 192897 Mouse
- Entrez Gene: 25724 Rat
- Omim: 147557 Human
- SwissProt: P16144 Human
- SwissProt: A2A863 Mouse
- SwissProt: Q64632 Rat
- Unigene: 632226 Human
see all -
Alternative names
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
Images
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Western blot - Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)All lanes : Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with absence of peptide
Lane 3 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1494) peptide
Lane 4 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1526) peptide
Predicted band size: 202 kDa
Datasheets and documents
Protocols
References
This product has been referenced in:
- Liu SY et al. A small molecule induces integrin ß4 nuclear translocation and apoptosis selectively in cancer cells with high expression of integrin ß4. Oncotarget 7:16282-96 (2016). Read more (PubMed: 26918348) »
