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I had a question regarding the products ab29044 and ab63546, both
antibodies for phosphorylated b4 integrin, but phosphorylated at
different sites. The tested application notes says that these have
been tested for use in ELISAs, and this is exactly what I wanted to
do. However, no further details are given - do you have the other
products I would need to run such an elisa? i.e. prepared elisa plates
or suggested capture antibodies and a protocol I could use to look for
both of these? Please let me know, thanks!
Asked on Sep 10 2012
Thanks for your email.
We don't have any additional experimental details beyone what is noted on the online product datasheets.
I have checked our catalog and unfortunately we don't have any beta 4 integrin ELISA kits available. Nonetheless I'm happy to help you choose antibodies and any other reagents you need that will help you set up your integrin beta 4 ELISA.
1. What species of integrin beta 4 will you be working with?
2. What sort of samples will you be using?
3. Do your samples contain small amounts of integrin beta 4 such that sensitivity is really criticial or do your samples express large amount of integrin beta 4?
4. Will you be comparing phosphorylated beta 4 integrin to total integrin?
Thanks for the additional details!
Answered on Sep 10 2012