Anti-Integrin beta 5 antibody (ab15459)
- Datasheet
- References (25)
- Protocols
Overview
-
Product name
Anti-Integrin beta 5 antibody
See all Integrin beta 5 primary antibodies -
Description
Rabbit polyclonal to Integrin beta 5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Hamster
-
Immunogen
Synthetic peptide within Human Integrin beta 5 aa 750 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P18084 -
Positive control
- Small intestine, bone marrow and colon carcinoma HeLa Cells Rat gastric epithelial
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab15459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/20. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22025551 | |
| IP | Use at an assay dependent concentration. PubMed: 22025551 | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3693 Human
- Entrez Gene: 16419 Mouse
- Entrez Gene: 257645 Rat
- Omim: 147561 Human
- SwissProt: P18084 Human
- SwissProt: O70309 Mouse
- Unigene: 536663 Human
- Unigene: 6424 Mouse
see all -
Alternative names
- FLJ26658 antibody
- Integrin beta-5 antibody
- Integrin subunit beta 5 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 5 antibody (ab15459)ICC/IF image of ab15459 stained HeLa cells (ab150035ab15459), 1µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody (ab15459) -
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 5 antibody (ab15459)This image is courtesy of an Abreview submitted by Dr J. Chaiab15459 staining Integrin beta 5 in Rat gastric epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 0.25% serum free protein for 20 minutes at 28°C. Samples were incubated with primary antibody (1/50 in antibody diluent) for 2 hours at 28°C. An FITC-conjugated Goat anti-IgG polyclonal (1/80) was used as the secondary antibody. Nuclei were counterstained with propidium iodide (ab14083).
Protocols
References
This product has been referenced in:
- Lin Z et al. Integrin-ß5, a miR-185-targeted gene, promotes hepatocellular carcinoma tumorigenesis by regulating ß-catenin stability. J Exp Clin Cancer Res 37:17 (2018). Read more (PubMed: 29386044) »
- Chai K et al. Heat shock protein 90 inhibitors augment endogenous wild-type p53 expression but down-regulate the adenovirally-induced expression by inhibiting a proteasome activity. Oncotarget 9:26130-26143 (2018). Read more (PubMed: 29899847) »
