Overview

  • Product name

    Anti-Integrin beta 5 antibody
    See all Integrin beta 5 primary antibodies
  • Description

    Goat polyclonal to Integrin beta 5
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    (C)TFNKFNKSYNGTVD

    , corresponding to C terminal amino acids 786-799 of Human Integrin beta 5

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    WB: 1/2000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). Can be blocked with Integrin beta 5 peptide (786-799) (ab45697).
    ELISA: This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:16,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-Integrin beta 5 antibody (ab31327) at 0.1 µg/ml + Tissue lysates prepared from Human Ovary at 35 µg

      Predicted band size: 88 kDa



      Primary incubation was for 1 hour. Detected by chemiluminescence

    References

    This product has been referenced in:

    • Dong H  et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). Read more (PubMed: 31366904) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your enquiry.

    The integrin beta 5protein is known to be glycosylated. Some other integrin beta 5 antibodies do show a ˜100 kDa and this is probably the glycosylated form.

    The following Uniprot protein database entry for integrin beta 5 has some information on the glycosylation sites:
    http://www.uniprot.org/uniprot/P18084

    Our source for this antibody has informed me they have also found 88 kDa band witha few tissues, but there was lots of breakdown signals. The human ovary and mouse eye lysates looked a lot cleaner. The post translationalglycoslyation and size of the band may therefore be different for individual tissues.

    I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (eyecup)
    Loading amount
    20 µg
    Specification
    eyecup
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    Submitted Jul 21 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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