RecombinantRabMAb

Recombinant Anti-Integrin beta 5 antibody [EPR19170] (ab184312)

Submit a review Submit a question References (1)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19170] to Integrin beta 5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin beta 5 antibody [EPR19170]
    See all Integrin beta 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR19170] to Integrin beta 5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse Integrin beta 5 aa 400-700. The exact sequence is proprietary.
    Database link: O70309

  • Positive control

    • WB: Rat and mouse lung lysates; A549, Neuro-2a, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19170

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).

Target

Images

  • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : Rat lung lysate at 20 µg
    Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : Mouse lung lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes; Lane 3: 1 minute; Lane 4: 3 minutes.

     

  • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2 and 3: 15 seconds; Lane 4: 1 minute

References (1)

Publishing research using ab184312? Please let us know so that we can cite the reference in this datasheet.

ab184312 has been referenced in 1 publication.

  • Chen J  et al. Remarkable Boron Delivery Of iRGD-Modified Polymeric Nanoparticles For Boron Neutron Capture Therapy. Int J Nanomedicine 14:8161-8177 (2019). PubMed: 31632025

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab184312.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com