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  1. Link

    integrin-beta-5-antibody-epr19170-ab184312.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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RecombinantRabMAb

Recombinant Anti-Integrin beta 5 antibody [EPR19170] (ab184312)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (4)

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Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
  • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
  • Anti-Integrin beta 5 antibody [EPR19170] (ab184312)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19170] to Integrin beta 5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Integrin beta 5 antibody [EPR19170]
    See all Integrin beta 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR19170] to Integrin beta 5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat and mouse lung lysates; A549, Neuro-2a, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19170
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta

Associated products

  • Alternative Versions

    • Anti-Integrin beta 5 antibody [EPR19170] - BSA and Azide free (ab240284)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
Notes
WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).

Target

  • Function

    Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.
  • Sequence similarities

    Belongs to the integrin beta chain family.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P18084 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3693 Human
    • Entrez Gene: 16419 Mouse
    • Entrez Gene: 257645 Rat
    • Omim: 147561 Human
    • SwissProt: P18084 Human
    • SwissProt: O70309 Mouse
    • Unigene: 536663 Human
    • Unigene: 6424 Mouse
    • Alternative names

      • FLJ26658 antibody
      • Integrin beta-5 antibody
      • Integrin subunit beta 5 antibody
      • integrin, beta 5 antibody
      • ITB5_HUMAN antibody
      • Itgb5 antibody
      • Testis secretory sperm-binding protein Li 217p antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
      Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
      All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

      Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
      Lane 2 : Rat lung lysate at 20 µg
      Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
      Lane 4 : Mouse lung lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 88 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes; Lane 3: 1 minute; Lane 4: 3 minutes.

       

    • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
      Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
      All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

      Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
      Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 88 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1, 2 and 3: 15 seconds; Lane 4: 1 minute

    • Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
      Anti-Integrin beta 5 antibody [EPR19170] (ab184312)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab184312? Please let us know so that we can cite the reference in this datasheet.

    ab184312 has been referenced in 4 publications.

    • Tang C  et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Commun Biol 4:161 (2021). PubMed: 33547385
    • Huang C  et al. RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin ß5 axis. Life Sci 267:118928 (2021). PubMed: 33358902
    • Kim SE  et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510
    • Chen J  et al. Remarkable Boron Delivery Of iRGD-Modified Polymeric Nanoparticles For Boron Neutron Capture Therapy. Int J Nanomedicine 14:8161-8177 (2019). PubMed: 31632025

    Customer reviews and Q&As

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