Recombinant
RabMAb

Recombinant Anti-Integrin beta 5 antibody [EPR19170] (ab184312)

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Overview

  • Product name

    Anti-Integrin beta 5 antibody [EPR19170]
    See all Integrin beta 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR19170] to Integrin beta 5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Mouse Integrin beta 5 aa 400-700. The exact sequence is proprietary.
    Database link: O70309

  • Positive control

    • WB: Rat and mouse lung lysates; A549, Neuro-2a, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19170

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).

Target

Images

  • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : Rat lung lysate at 20 µg
    Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : Mouse lung lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes; Lane 3: 1 minute; Lane 4: 3 minutes.

     

  • Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
    All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 88 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2 and 3: 15 seconds; Lane 4: 1 minute

References

ab184312 has not yet been referenced specifically in any publications.

Publishing research using ab184312? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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