Recombinant Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
![Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)](https://www.abcam.com/ps/products/184/ab184312/Images/ab184312-276168-anti-integrin-beta-5-antibody-epr19170-western-blot.jpg "Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19170] to Integrin beta 5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin beta 5 antibody [EPR19170]
See all Integrin beta 5 primary antibodies -
Description
Rabbit monoclonal [EPR19170] to Integrin beta 5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat and mouse lung lysates; A549, Neuro-2a, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19170 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa). |
Target
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Function
Integrin alpha-V/beta-5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3693 Human
- Entrez Gene: 16419 Mouse
- Entrez Gene: 257645 Rat
- Omim: 147561 Human
- SwissProt: P18084 Human
- SwissProt: O70309 Mouse
- Unigene: 536663 Human
- Unigene: 6424 Mouse
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Alternative names
- FLJ26658 antibody
- Integrin beta-5 antibody
- Integrin subunit beta 5 antibody
see all
Images
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Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2 : Rat lung lysate at 20 µg
Lane 3 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 4 : Mouse lung lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes; Lane 3: 1 minute; Lane 4: 3 minutes.
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![Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)](https://www.abcam.com/ps/products/184/ab184312/Images/ab184312-276167-anti-integrin-beta-5-antibody-epr19170-western-blot.jpg)
Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312)All lanes : Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1, 2 and 3: 15 seconds; Lane 4: 1 minute
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![Anti-Integrin beta 5 antibody [EPR19170] (ab184312)](https://www.abcam.com/ps/products/184/ab184312/Images/ab184312-3-benefits-of-recombinant-antibodies.png)
Anti-Integrin beta 5 antibody [EPR19170] (ab184312)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab184312 has been referenced in 4 publications.
- Tang C et al. A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface. Commun Biol 4:161 (2021). PubMed: 33547385
- Huang C et al. RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin ß5 axis. Life Sci 267:118928 (2021). PubMed: 33358902
- Kim SE et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510
- Chen J et al. Remarkable Boron Delivery Of iRGD-Modified Polymeric Nanoparticles For Boron Neutron Capture Therapy. Int J Nanomedicine 14:8161-8177 (2019). PubMed: 31632025
