Anti-Integrin beta 7 antibody (ab169300)
Key features and details
- Mouse polyclonal to Integrin beta 7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin beta 7 antibody
See all Integrin beta 7 primary antibodies -
Description
Mouse polyclonal to Integrin beta 7 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset
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Immunogen
Full length protein, corresponding to amino acids 1-798 of Human Integrin beta 7 (NP_000880.1).
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Positive control
- Integrin beta 7 transfected 293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab169300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 87 kDa. |
Target
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Function
Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin. -
Tissue specificity
Expressed in a variety of leukocyte lines. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Domain
Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 451934 Chimpanzee
- Entrez Gene: 100402863 Common marmoset
- Entrez Gene: 514031 Cow
- Entrez Gene: 101145271 Gorilla
- Entrez Gene: 100063747 Horse
- Entrez Gene: 3695 Human
- Entrez Gene: 16421 Mouse
- Entrez Gene: 100626600 Pig
see all -
Alternative names
- Gut homing receptor beta subunit antibody
- Integrin beta 7 subunit antibody
- Integrin beta-7 antibody
see all
Images
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Western blot - Anti-Integrin beta 7 antibody (ab169300)All lanes : Anti-Integrin beta 7 antibody (ab169300) at 1 µg/ml
Lane 1 : Integrin beta 7 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Datasheets and documents
References (0)
ab169300 has not yet been referenced specifically in any publications.
