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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Beta
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Anti-Integrin beta 7 antibody (ab169300)

  • Datasheet
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Western blot - Anti-Integrin beta 7 antibody (ab169300)

    Key features and details

    • Mouse polyclonal to Integrin beta 7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Integrin beta 7 protein (ab114397)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-Integrin beta 7 antibody
      See all Integrin beta 7 primary antibodies
    • Description

      Mouse polyclonal to Integrin beta 7
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rabbit, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset
    • Immunogen

      Full length protein, corresponding to amino acids 1-798 of Human Integrin beta 7 (NP_000880.1).

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Integrin beta 7 transfected 293T cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.4
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Beta
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG - Isotype Control (ab37355)
    • Recombinant Protein

      • Recombinant Human Integrin beta 7 protein (ab114397)
    • Related Products

      • Recombinant Human Integrin beta 7 protein (ab114397)

    Applications

    Our Abpromise guarantee covers the use of ab169300 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Predicted molecular weight: 87 kDa.

    Target

    • Function

      Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
    • Tissue specificity

      Expressed in a variety of leukocyte lines.
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Domain

      Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P26010 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 451934 Chimpanzee
      • Entrez Gene: 100402863 Common marmoset
      • Entrez Gene: 514031 Cow
      • Entrez Gene: 101145271 Gorilla
      • Entrez Gene: 100063747 Horse
      • Entrez Gene: 3695 Human
      • Entrez Gene: 16421 Mouse
      • Entrez Gene: 100626600 Pig
      • Entrez Gene: 699405 Rhesus monkey
      • Omim: 147559 Human
      • SwissProt: P26010 Human
      • SwissProt: P26011 Mouse
      • Unigene: 654470 Human
      • Unigene: 58 Mouse
      see all
    • Alternative names

      • Gut homing receptor beta subunit antibody
      • Integrin beta 7 subunit antibody
      • Integrin beta-7 antibody
      • Integrin beta-P antibody
      • Integrin, beta 7 antibody
      • ITB7 antibody
      • ITB7_HUMAN antibody
      • Itgb7 antibody
      • Ly69 antibody
      • M290 IEL antigen antibody
      see all

    Images

    • Western blot - Anti-Integrin beta 7 antibody (ab169300)
      Western blot - Anti-Integrin beta 7 antibody (ab169300)
      All lanes : Anti-Integrin beta 7 antibody (ab169300) at 1 µg/ml

      Lane 1 : Integrin beta 7 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 87 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab169300? Please let us know so that we can cite the reference in this datasheet.

    ab169300 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin beta 7 antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (intestine)
    Permeabilization
    No
    Specification
    intestine
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Dr. Abdul Rauf

    Verified customer

    Submitted Sep 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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