Key features and details
- Rabbit polyclonal to Integrin beta 7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Integrin beta 7 antibody
See all Integrin beta 7 primary antibodies
DescriptionRabbit polyclonal to Integrin beta 7
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Integrin beta 7. (Near the N terminal; NP_000880).
Database link: P26010
- PC3 and K562 cell lysates. Human gastric cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/200 - 1/1000. Predicted molecular weight: 87 kDa.|
FunctionIntegrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
Tissue specificityExpressed in a variety of leukocyte lines.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
DomainDomain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
- Information by UniProt
- Gut homing receptor beta subunit antibody
- Integrin beta 7 subunit antibody
- Integrin beta-7 antibody
All lanes : Anti-Integrin beta 7 antibody (ab198796) at 1/200 dilution
Lane 1 : PC3 cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 87 kDa
Exposure time: 10 minutes
Gel: 6% SDS-PAGE.
Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Integrin beta 7 with ab198796 at 1/30 dilution.
ab198796 has not yet been referenced specifically in any publications.