Recombinant
RabMAb

Recombinant Anti-Integrin beta 7 antibody [EP5948] (ab137058)

Overview

  • Product name

    Anti-Integrin beta 7 antibody [EP5948]
    See all Integrin beta 7 primary antibodies
  • Description

    Rabbit monoclonal [EP5948] to Integrin beta 7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acids from the N terminal of Human Integrin beta 7 (UniProt ID: P26010).

  • Positive control

    • WB: IM-9 cell lysate. Flow Cyt: Jurkat cells. ICC/IF: IM-9 cells.
  • General notes

     

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
    • Tissue specificity

      Expressed in a variety of leukocyte lines.
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Domain

      Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Gut homing receptor beta subunit antibody
      • Integrin beta 7 subunit antibody
      • Integrin beta-7 antibody
      • Integrin beta-P antibody
      • Integrin, beta 7 antibody
      • ITB7 antibody
      • ITB7_HUMAN antibody
      • Itgb7 antibody
      • Ly69 antibody
      • M290 IEL antigen antibody
      see all

    Images

    • Anti-Integrin beta 7 antibody [EP5948] (ab137058) at 1/1000 dilution + IM-9 cell lysate at 10 µg

      Secondary
      HRP labeled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 87 kDa

    References

    ab137058 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Fixation
    unfixed
    Permeabilization
    No
    Sample
    Human Cell (Jurkat T cell)
    Specification
    Jurkat T cell
    Gating Strategy
    by scatter profile, Jurkat
    Preparation
    Cell harvesting/tissue preparation method: cell line
    Sample buffer: DMEM

    Abcam user community

    Verified customer

    Submitted Aug 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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