Recombinant
RabMAb

Recombinant Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free (ab216688)

Overview

  • Product name

    Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free
    See all Integrin beta 7 primary antibodies
  • Description

    Rabbit monoclonal [EP5948] to Integrin beta 7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Integrin beta 7 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P26010

  • Positive control

    • WB: IM-9 cell lysate. Flow Cyt: Jurkat cells. ICC/IF: IM-9 cells.
  • General notes

    Ab216688 is the carrier-free version of ab137058. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab216688 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 87 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.
    • Tissue specificity

      Expressed in a variety of leukocyte lines.
    • Sequence similarities

      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Domain

      Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Gut homing receptor beta subunit antibody
      • Integrin beta 7 subunit antibody
      • Integrin beta-7 antibody
      • Integrin beta-P antibody
      • Integrin, beta 7 antibody
      • ITB7 antibody
      • ITB7_HUMAN antibody
      • Itgb7 antibody
      • Ly69 antibody
      • M290 IEL antigen antibody
      see all

    References

    ab216688 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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