Product nameAnti-Integrin beta 8 antibody
See all Integrin beta 8 primary antibodies
DescriptionRabbit polyclonal to Integrin beta 8
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
- Human placenta membrane lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab80673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Only Peptide ELISA tested
|IHC-P||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionIntegrin alpha-V/beta-8 is a receptor for fibronectin.
Tissue specificityPlacenta, kidney, brain, ovary, uterus and in several transformed cells. Transiently expressed in 293 human embryonic kidney cells.
Sequence similaritiesBelongs to the integrin beta chain family.
Contains 1 VWFA domain.
- Information by UniProt
- Integrin beta-8 antibody
- ITB8_HUMAN antibody
- ITGB 8 antibody
- ITGB8 antibody
IHC image of Integrin beta 8 staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80673, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Anti-Integrin beta 8 antibody (ab80673) at 1 µg/ml + Human Placenta lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Observed band size: 81 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Greenhalgh SN et al. Loss of Integrin avß8 in Murine Hepatocytes Accelerates Liver Regeneration. Am J Pathol 189:258-271 (2019). Read more (PubMed: 30448409) »
- Jimenez L et al. Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) Reveals Enrichment of Adhesion Proteins in Small EVs. J Proteome Res 18:947-959 (2019). Read more (PubMed: 30608700) »