Overview

  • Product name
    Anti-Integrin beta antibody [EPR20825]
    See all Integrin beta primary antibodies
  • Description
    Rabbit monoclonal [EPR20825] to Integrin beta
  • Host species
    Rabbit
  • Specificity

    The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Integrin beta 3 aa 1-400. The exact sequence is proprietary.
    Database link: P05106

  • Positive control
    • WB: HeLa, C2C12, HUVEC and C6 whole cell lysates; Human fetal spleen lysate; Mouse kidney lysate; Rat spleen, heart and liver lysates. IHC-P: Human, mouse and rat spleen tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/20000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance
    Disease: Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. Function: Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. PTM: Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. Similarity: Belongs to the integrin beta chain family. Contains 1 VWFA domain. Tissue specificity: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
  • Database links
  • Alternative names
    • Integrin beta-1 antibody
    • Integrin beta-2 antibody
    • Integrin beta-3 antibody
    • Integrin beta-4 antibody
    • Integrin beta-5 antibody
    • Integrin beta-6 antibody
    • Integrin beta-7 antibody
    • Integrin beta-8 antibody
    see all

Images

  • All lanes : Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
    Lane 5 : Human fetal spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1-3: 3 minutes; Lane 4: 15 seconds; Lane 5: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9610392).

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets of human spleen (PMID: 22434870). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

  • All lanes : Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution or Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500

    Lane 1 : U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 2 : HEL (Human Erythroleukemia erythroblast) whole cell lysates with 5% NFDM/TBST
    Lane 3 : LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 5 : HUVEC (Human umbilical vein endothelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 6 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
    Lane 7 : C2C12 (Mouse myoblasts myoblast) whole cell lysates with 5% NFDM/TBST
    Lane 8 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?



    Comparison of staining with an antibody specific to Integrin beta 3 (ab119992).

    The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

     

    Exposure:

    For ab210515: 1, 2, 5 lanes: 3 s; 3, 4 lanes: 37 s; 6 lane: 3 min; 7 lane: 15 s; 8 lane: 114 s. 

    For ab119992: 1, 2 lane: 10 s; 3~8 lanes: 3 min

  • All lanes : Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 3 : Rat heart lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Exposure time : Lane 1: 30 seconds; Lane 2: 3 seconds; Lane 3: 3 minutes; Lane 4: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 9610392).

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of mouse spleen (PMID: 22434870). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of rat spleen (PMID: 22434870). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

References

This product has been referenced in:
  • Shang C  et al. The human platelet transcriptome and proteome is altered and pro-thrombotic functional responses are increased during prolonged hypoxia exposure at high altitude. Platelets N/A:1-10 (2019). Read more (PubMed: 30721642) »
See 1 Publication for this product

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