Anti-Integrin linked ILK antibody (ab217025)
Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies -
Description
Rabbit polyclonal to Integrin linked ILK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Integrin linked ILK aa 300-400 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13418 -
Positive control
- Rat kidney tissue; human colon carcinoma lysate; A549 and MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200. Predicted molecular weight: 51 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/200. Predicted molecular weight: 51 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
Post-translational
modificationsAutophosphorylated on serine residues. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all -
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Images
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All lanes : Anti-Integrin linked ILK antibody (ab217025) at 1/200 dilution
Lane 1 : rat kidney lysates
Lane 2 : human colon carcinoma lysates
Secondary
All lanes : Conjugated secondary antibody at 1/3000 dilution
Predicted band size: 51 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin embedded rat kidney tissue labeling Integrin linked ILK with ab217025 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunofluorescent analysis of MCF7 cells labeling Integrin linked ILK with ab217025 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody detection.
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Immunofluorescent analysis of A549 cells labeling Integrin linked ILK with ab217025 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody detection.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab217025 has not yet been referenced specifically in any publications.