Overview

  • Product name

    Anti-Integrin linked ILK antibody
    See all Integrin linked ILK primary antibodies
  • Description

    Rabbit polyclonal to Integrin linked ILK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Integrin linked ILK aa 340-390 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    VKFSFQCPGRM YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV


    Database link: Q13418

  • Positive control

    • Rat kidney tissue; human colon carcinoma lysate; A549 and MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • Tissue specificity

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • Domain

    A PH-like domain is involved in phosphatidylinositol phosphate binding.
  • Post-translational
    modifications

    Autophosphorylated on serine residues.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images

  • All lanes : Anti-Integrin linked ILK antibody (ab217025) at 1/200 dilution

    Lane 1 : rat kidney lysates
    Lane 2 : human colon carcinoma lysates

    Secondary
    All lanes : Conjugated secondary antibody at 1/3000 dilution

    Predicted band size: 51 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded rat kidney tissue labeling Integrin linked ILK with ab217025 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

  • Immunofluorescent analysis of MCF7 cells labeling Integrin linked ILK with ab217025 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody detection.

  • Immunofluorescent analysis of A549 cells labeling Integrin linked ILK with ab217025 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody detection.

References

ab217025 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab217025.
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