Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies
DescriptionRabbit polyclonal to Integrin linked ILK
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat kidney tissue; human colon carcinoma lysate; A549 and MCF7 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
DomainA PH-like domain is involved in phosphatidylinositol phosphate binding.
modificationsAutophosphorylated on serine residues.
Cellular localizationCell junction > focal adhesion. Cell membrane.
- Information by UniProt
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
All lanes : Anti-Integrin linked ILK antibody (ab217025) at 1/200 dilution
Lane 1 : rat kidney lysates
Lane 2 : human colon carcinoma lysates
All lanes : Conjugated secondary antibody at 1/3000 dilution
Predicted band size: 51 kDa
Immunohistochemical analysis of formalin-fixed and paraffin embedded rat kidney tissue labeling Integrin linked ILK with ab217025 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunofluorescent analysis of MCF7 cells labeling Integrin linked ILK with ab217025 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody detection.
Immunofluorescent analysis of A549 cells labeling Integrin linked ILK with ab217025 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody detection.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab217025 has not yet been referenced specifically in any publications.