Anti-Integrin linked ILK antibody (ab227154)

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Key features and details

  • Rabbit polyclonal to Integrin linked ILK
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Integrin linked ILK antibody
    See all Integrin linked ILK primary antibodies

  • Description

    Rabbit polyclonal to Integrin linked ILK

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Guinea pig, Cow, Rhesus monkey

  • Immunogen

    Recombinant fragment within Human Integrin linked ILK (internal sequence). The exact sequence is proprietary.
    Database link: Q13418

  • Positive control

    • WB: WT HeLa cell extracts. PC-12, Neuro-2a, C8D80, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. A431 whole cell lysate. IHC-P: Mouse kidney tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

  • Tissue specificity

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.

  • Domain

    A PH-like domain is involved in phosphatidylinositol phosphate binding.

  • Post-translational
    modifications

    Autophosphorylated on serine residues.

  • Cellular localization

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images

  • Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Western blot - Anti-Integrin linked ILK antibody (ab227154)
    All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Integrin linked ILK Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 51 kDa

  • Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Western blot - Anti-Integrin linked ILK antibody (ab227154)
    All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

    Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
    Lane 2 : Integrin linked ILK knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (ab227154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (ab227154)

    Paraffin embedded mouse kidney tissue stained for Integrin linked ILK with ab227154 (1/500 dilution) in immunohistochemical analysis.

  • Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Western blot - Anti-Integrin linked ILK antibody (ab227154)
    All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
    Lane 2 : C3D80 whole cell extracts
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Immunoprecipitation - Anti-Integrin linked ILK antibody (ab227154)
    Immunoprecipitation - Anti-Integrin linked ILK antibody (ab227154)

    Integrin linked ILK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 µg of ab227154. Western blot was performed from the immunoprecipitate using ab227154 at1/500 dilution.

    Lane 1: 30 μg HeLa whole cell extract

    Lane 2: Control with 4 μg of preimmune Rabbit IgG.

    Lane 3: ab227154 IP in HeLa whole cell extracts.

    10% SDS-PAGE.

  • Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    7.5% SDS PAGE

  • Western blot - Anti-Integrin linked ILK antibody (ab227154)
    Western blot - Anti-Integrin linked ILK antibody (ab227154)
    All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C3D80 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10 % SDS-PAGE

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab227154? Please let us know so that we can cite the reference in this datasheet.

ab227154 has been referenced in 1 publication.

  • Zuo Z  et al. Acupuncture attenuates renal interstitial fibrosis via the TGF-ß/Smad pathway. Mol Med Rep 20:2267-2275 (2019). PubMed: 31322212

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