Anti-Integrin linked ILK antibody (ab227154)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies -
Description
Rabbit polyclonal to Integrin linked ILK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Guinea pig, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human Integrin linked ILK (internal sequence). The exact sequence is proprietary.
Database link: Q13418 -
Positive control
- WB: WT HeLa cell extracts. PC-12, Neuro-2a, C8D80, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. A431 whole cell lysate. IHC-P: Mouse kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 51 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
Post-translational
modificationsAutophosphorylated on serine residues. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 540207 Cow
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q3SWY2 Cow
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
see all -
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Images
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Western blot - Anti-Integrin linked ILK antibody (ab227154)All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Integrin linked ILK Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa -
Western blot - Anti-Integrin linked ILK antibody (ab227154)All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : Integrin linked ILK knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (ab227154)Paraffin embedded mouse kidney tissue stained for Integrin linked ILK with ab227154 (1/500 dilution) in immunohistochemical analysis.
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Western blot - Anti-Integrin linked ILK antibody (ab227154)All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
Lane 2 : C3D80 whole cell extracts
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Immunoprecipitation - Anti-Integrin linked ILK antibody (ab227154)Integrin linked ILK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 µg of ab227154. Western blot was performed from the immunoprecipitate using ab227154 at1/500 dilution.
Lane 1: 30 μg HeLa whole cell extract
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227154 IP in HeLa whole cell extracts.
10% SDS-PAGE.
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Western blot - Anti-Integrin linked ILK antibody (ab227154)Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa10% SDS-PAGE
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Western blot - Anti-Integrin linked ILK antibody (ab227154)Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa7.5% SDS PAGE
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Western blot - Anti-Integrin linked ILK antibody (ab227154)All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C3D80 whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa10 % SDS-PAGE
References
ab227154 has not yet been referenced specifically in any publications.
