Overview

  • Product name

    Anti-Integrin linked ILK antibody
    See all Integrin linked ILK primary antibodies
  • Description

    Rabbit polyclonal to Integrin linked ILK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Guinea pig, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Integrin linked ILK (internal sequence). The exact sequence is proprietary.
    Database link: Q13418

  • Positive control

    • WB: WT HeLa cell extracts. PC-12, Neuro-2a, C8D80, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. A431 whole cell lysate. IHC-P: Mouse kidney tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • Tissue specificity

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • Domain

    A PH-like domain is involved in phosphatidylinositol phosphate binding.
  • Post-translational
    modifications

    Autophosphorylated on serine residues.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images

  • All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Integrin linked ILK Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 51 kDa

  • All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

    Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
    Lane 2 : Integrin linked ILK knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Paraffin embedded mouse kidney tissue stained for Integrin linked ILK with ab227154 (1/500 dilution) in immunohistochemical analysis.

  • All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extracts
    Lane 2 : C3D80 whole cell extracts
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Integrin linked ILK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 µg of ab227154. Western blot was performed from the immunoprecipitate using ab227154 at1/500 dilution.

    Lane 1: 30 μg HeLa whole cell extract

    Lane 2: Control with 4 μg of preimmune Rabbit IgG.

    Lane 3: ab227154 IP in HeLa whole cell extracts.

    10% SDS-PAGE.

  • Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    7.5% SDS PAGE

  • All lanes : Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C3D80 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10 % SDS-PAGE

References

ab227154 has not yet been referenced specifically in any publications.

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