Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: ICC, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Product nameAnti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies
DescriptionRabbit polyclonal to Integrin linked ILK
Tested applicationsSuitable for: ICC, IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Recombinant fragment corresponding to Human Integrin linked ILK aa 1-228.
Database link: Q13418
- WB: HeLa, NIH/3T3 and HL-60 whole cell lysates; Mouse liver and lung lysates; Rat liver and lung lysates; Zebrafish lysate. IHC-P: Human kidney and skeletal muscle tissue; paraffin-embedded HEK-293T cells. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 51 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
DomainA PH-like domain is involved in phosphatidylinositol phosphate binding.
modificationsAutophosphorylated on serine residues.
Cellular localizationCell junction > focal adhesion. Cell membrane.
- Information by UniProt
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
All lanes : Anti-Integrin linked ILK antibody (ab236455) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : Mouse liver lysate
Lane 5 : Mouse lung lysate
Lane 6 : Rat liver lysate
Lane 7 : Rat lung lysate
Lane 8 : Zebrafish lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
Paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Integrin linked ILK (green) using ab236455 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236455 has not yet been referenced specifically in any publications.