Overview

  • Product name

    Anti-Integrin linked ILK antibody
    See all Integrin linked ILK primary antibodies
  • Description

    Rabbit polyclonal to Integrin linked ILK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human Integrin linked ILK aa 1-228.
    Database link: Q13418

  • Positive control

    • WB: HeLa, NIH/3T3 and HL-60 whole cell lysates; Mouse liver and lung lysates; Rat liver and lung lysates; Zebrafish lysate. IHC-P: Human kidney and skeletal muscle tissue; paraffin-embedded HEK-293T cells. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100.
IHC-P 1/20 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 51 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • Tissue specificity

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • Domain

    A PH-like domain is involved in phosphatidylinositol phosphate binding.
  • Post-translational
    modifications

    Autophosphorylated on serine residues.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images

  • All lanes : Anti-Integrin linked ILK antibody (ab236455) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
    Lane 4 : Mouse liver lysate
    Lane 5 : Mouse lung lysate
    Lane 6 : Rat liver lysate
    Lane 7 : Rat lung lysate
    Lane 8 : Zebrafish lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 51 kDa

  • Paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney tissue stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human skeletal muscle tissue stained for Integrin linked ILK using ab236455 at 1/100 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Integrin linked ILK (green) using ab236455 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236455 has not yet been referenced specifically in any publications.

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