Key features and details
- Rabbit polyclonal to Integrin linked ILK
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Integrin linked ILK antibody
See all Integrin linked ILK primary antibodies
DescriptionRabbit polyclonal to Integrin linked ILK
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish
Synthetic peptide corresponding to a region between residues 402 and 451 of human Integrin linked ILK (NP_004508.1)
- HeLa, 293T and NIH3T3 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74336 was affinity purified using an epitope specific to Integrin linked ILK immobilized on solid support.
Our Abpromise guarantee covers the use of ab74336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 51 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Tissue specificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain.
DomainA PH-like domain is involved in phosphatidylinositol phosphate binding.
modificationsAutophosphorylated on serine residues.
Cellular localizationCell junction > focal adhesion. Cell membrane.
- Information by UniProt
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
All lanes : Anti-Integrin linked ILK antibody (ab74336) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 10 seconds
Immunocytochemistry/Immunofluorescence analysis of acetone-fixed asynchronous HeLa cells labelling Integrin linked ILK with ab74336 at 1/100 (2µg/ml). A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
Detection of Integrin linked ILK by Western Blot of Immunprecipitate.
ab74336 at 1µg/ml staining Integrin linked ILK in HeLa whole cell lysate immunoprecipitated using ab74336 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ICC/IF image of ab74336 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74336, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74336 has been referenced in 2 publications.
- Manak MS et al. Live-cell phenotypic-biomarker microfluidic assay for the risk stratification of cancer patients via machine learning. Nat Biomed Eng 2:761-772 (2018). PubMed: 30854249
- Lin F et al. Echistatin prevents posterior capsule opacification in diabetic rabbit model via integrin linked kinase signaling pathway. Int J Clin Exp Pathol 8:14294-304 (2015). PubMed: 26823745