Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Integrin linked ILK antibody [EPR1592] - BSA and Azide free (ab239884)

Overview

  • Product name

    Anti-Integrin linked ILK antibody [EPR1592] - BSA and Azide free
    See all Integrin linked ILK primary antibodies
  • Description

    Rabbit monoclonal [EPR1592] to Integrin linked ILK - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Integrin linked ILK. The exact sequence is proprietary.

  • General notes

    Ab239884 is the carrier-free version of ab76468. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239884 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1592
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab239884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
    • Tissue specificity

      Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 5 ANK repeats.
      Contains 1 protein kinase domain.
    • Domain

      A PH-like domain is involved in phosphatidylinositol phosphate binding.
    • Post-translational
      modifications

      Autophosphorylated on serine residues.
    • Cellular localization

      Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 59 kDa serine/threonine protein kinase antibody
      • 59 kDa serine/threonine-protein kinase antibody
      • DKFZp686F1765 antibody
      • Epididymis secretory protein Li 28 antibody
      • HEL S 28 antibody
      • ILK 1 antibody
      • ILK 2 antibody
      • ILK antibody
      • ILK-1 antibody
      • ILK-2 antibody
      • ILK_HUMAN antibody
      • ILK1 antibody
      • ILK2 antibody
      • Integrin linked kinase 2 antibody
      • Integrin linked Kinase antibody
      • Integrin linked protein kinase antibody
      • Integrin-linked protein kinase antibody
      • p59 antibody
      • p59ILK antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Integrin linked ILK (green) with purified ab76468 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76468).

    • ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76468).

    • ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76468).

    • Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76468).

    References

    ab239884 has not yet been referenced specifically in any publications.

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