Recombinant
RabMAb

Recombinant Anti-Integrin linked ILK antibody [EPR1592] (Phycoerythrin) (ab211694)

Overview

  • Product name

    Anti-Integrin linked ILK antibody [EPR1592] (Phycoerythrin)
    See all Integrin linked ILK primary antibodies
  • Description

    Rabbit monoclonal [EPR1592] to Integrin linked ILK (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Integrin linked ILK aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: Q13418

  • Positive control

    • Flow Cyt: A431 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1592
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.

The cellular localisation of this product has been verified in ICC/IF

Target

  • Function

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • Tissue specificity

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • Domain

    A PH-like domain is involved in phosphatidylinositol phosphate binding.
  • Post-translational
    modifications

    Autophosphorylated on serine residues.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images

  • Overlay histogram showing A431 cells stained with ab211694 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab211694, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in A431 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab211694 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211694.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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