Anti-Integrin linked ILK (phospho S246) antibody (ab111435)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Integrin linked ILK (phospho S246) antibody
See all Integrin linked ILK primary antibodies -
DescriptionRabbit polyclonal to Integrin linked ILK (phospho S246)
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Host speciesRabbit
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Specificityab111435 detects endogenous levels of Integrin linked ILK only when phosphorylated at serine 246.
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from Human Integrin linked ILK around the phosphorylation site of Serine 246 (I-F-SP-H-P).
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Positive control
- Human brain tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab111435 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab111435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
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Tissue specificityHighly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
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Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
DomainA PH-like domain is involved in phosphatidylinositol phosphate binding.
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Post-translational
modificationsAutophosphorylated on serine residues. -
Cellular localizationCell junction > focal adhesion. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all -
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK (phospho S246) antibody (ab111435)ab111435, at 1/50 dilution, staining Integrin linked ILK in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
References
ab111435 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"