Anti-Integrin linked ILK (phospho S246) antibody (ab111435)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK (phospho S246) antibody (ab111435)

    Key features and details

    • Rabbit polyclonal to Integrin linked ILK (phospho S246)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Integrin linked ILK (phospho S246) antibody
      See all Integrin linked ILK primary antibodies

    • Description

      Rabbit polyclonal to Integrin linked ILK (phospho S246)

    • Host species

      Rabbit

    • Specificity

      ab111435 detects endogenous levels of Integrin linked ILK only when phosphorylated at serine 246.

    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data

    • Immunogen

      Synthetic phosphopeptide derived from Human Integrin linked ILK around the phosphorylation site of Serine 246 (I-F-SP-H-P).

    • Positive control

      • Human brain tissue

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab111435 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab111435 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

    • Tissue specificity

      Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

    • Sequence similarities

      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 5 ANK repeats.
      Contains 1 protein kinase domain.

    • Domain

      A PH-like domain is involved in phosphatidylinositol phosphate binding.

    • Post-translational
      modifications

      Autophosphorylated on serine residues.

    • Cellular localization

      Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 59 kDa serine/threonine protein kinase antibody
      • 59 kDa serine/threonine-protein kinase antibody
      • DKFZp686F1765 antibody
      • Epididymis secretory protein Li 28 antibody
      • HEL S 28 antibody
      • ILK 1 antibody
      • ILK 2 antibody
      • ILK antibody
      • ILK-1 antibody
      • ILK-2 antibody
      • ILK_HUMAN antibody
      • ILK1 antibody
      • ILK2 antibody
      • Integrin linked kinase 2 antibody
      • Integrin linked Kinase antibody
      • Integrin linked protein kinase antibody
      • Integrin-linked protein kinase antibody
      • p59 antibody
      • p59ILK antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK (phospho S246) antibody (ab111435)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK (phospho S246) antibody (ab111435)
      ab111435, at 1/50 dilution, staining Integrin linked ILK in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

    References (0)

    Publishing research using ab111435? Please let us know so that we can cite the reference in this datasheet.

    ab111435 has not yet been referenced specifically in any publications.

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