Anti-Integrin linked ILK (phospho S246) antibody (ab111435)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Integrin linked ILK (phospho S246) antibody
See all Integrin linked ILK primary antibodies -
Description
Rabbit polyclonal to Integrin linked ILK (phospho S246) -
Host species
Rabbit -
Specificity
ab111435 detects endogenous levels of Integrin linked ILK only when phosphorylated at serine 246. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from Human Integrin linked ILK around the phosphorylation site of Serine 246 (I-F-SP-H-P).
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Positive control
- Human brain tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab111435 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab111435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
Post-translational
modificationsAutophosphorylated on serine residues. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all -
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK (phospho S246) antibody (ab111435)ab111435, at 1/50 dilution, staining Integrin linked ILK in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
References
ab111435 has not yet been referenced specifically in any publications.
