Anti-Integrin linked ILK (phospho S246) antibody (ab111435)
Key features and details
- Rabbit polyclonal to Integrin linked ILK (phospho S246)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin linked ILK (phospho S246) antibody
See all Integrin linked ILK primary antibodies -
Description
Rabbit polyclonal to Integrin linked ILK (phospho S246) -
Host species
Rabbit -
Specificity
ab111435 detects endogenous levels of Integrin linked ILK only when phosphorylated at serine 246. -
Tested Applications & Species
Application Species IHC-P Human -
Immunogen
Synthetic phosphopeptide derived from Human Integrin linked ILK around the phosphorylation site of Serine 246 (I-F-SP-H-P).
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Positive control
- Human brain tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111435 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. -
Tissue specificity
Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 5 ANK repeats.
Contains 1 protein kinase domain. -
Domain
A PH-like domain is involved in phosphatidylinositol phosphate binding. -
Post-translational
modificationsAutophosphorylated on serine residues. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3611 Human
- Entrez Gene: 16202 Mouse
- Entrez Gene: 170922 Rat
- Omim: 602366 Human
- SwissProt: Q13418 Human
- SwissProt: O55222 Mouse
- SwissProt: Q99J82 Rat
- Unigene: 5158 Human
see all -
Alternative names
- 59 kDa serine/threonine protein kinase antibody
- 59 kDa serine/threonine-protein kinase antibody
- DKFZp686F1765 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK (phospho S246) antibody (ab111435)ab111435, at 1/50 dilution, staining Integrin linked ILK in paraffin-embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111435 has not yet been referenced specifically in any publications.