Anti-Interferon alpha 1 antibody (ab24767)
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Overview
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Product nameAnti-Interferon alpha 1 antibody
See all Interferon alpha 1 primary antibodies -
DescriptionRabbit polyclonal to Interferon alpha 1
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Host speciesRabbit
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SpecificityRecognizes chicken Interferon alpha 1 (chIFN-alpha). The antibody cross reacts with IFN beta but does not cross react with IFN gamma. This antibody will recognize chicken IFN beta by Western blotting; it does not neutralize its activity in vitro. It does, however, neutralize the activity of IFN alpha, and thus it can be used to distinguish the two bioactivities. Presumably it recognizes an epitope that is only revealed in IFN beta following denaturation.
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Tested applicationsSuitable for: ELISA, IP, WB, Functional Studiesmore details
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Species reactivityReacts with: Chicken
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Immunogen
Recombinant full length protein corresponding to Chicken Interferon alpha 1.
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General notes
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Concentration information loading...
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PurityProtein G purified
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Primary antibody notesThe classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab24767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | Use at an assay dependent dilution. | |
IP | Use at an assay dependent dilution. | |
WB | Use at an assay dependent dilution. Detects a band of approximately 19 kDa. | |
Functional Studies | Use at an assay dependent dilution. |
Target
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FunctionProduced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
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Sequence similaritiesBelongs to the alpha/beta interferon family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 396398 Chicken
- SwissProt: P42165 Chicken
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Alternative names
- IFL antibody
- IFN alpha antibody
- IFN alpha 1/13 antibody
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Datasheets and documents
References
ab24767 has not yet been referenced specifically in any publications.