Anti-Interferon alpha 1 antibody (ab24767)

Thank you for contacting us.  We do not test the concentration of the specific antibody in our protein G affinity purified antibodies. The 0.5 mg/mL concentration would correspond to total Ig. However, we expect >90% of the antibody to be specific in this type of preparation. The details of the neutralization assay are as follows: Second passage chicken embryo fibroblasts were induced with ChIFN with 1% rabbit antiserum. 15 hours post-induction, cells were infected with VSV. Inoculum was removed after 30 min by washing with PBS. Culture supernatant was harvested 12h post-infection. Virus titer determined.   I hope this helps, please let me know if you need any additional information.   

Thank you for your inquiry. Generally with affinity purified IgG, we expect >90% specific antibody. We do not measure the specific IgG concentration for these affinity purified products, just total IgG. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

Thank you for your enquiry. The situation with this antibody is that while it will recognize chicken interferon beta by Western blotting, it does not neutralize its activity in vitro. It does, however, neutralize the activity of interferon alpha, and thus it can be used to distinguish the two bioactivities. Presumably it recognizes an epitope that is only revealed in interferon beta following denaturation. The following reference is useful for examining the bioactivity of this antibody: Schwarz, H. et al. (2004). Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay. J. Interferon Cytokine Res. 24: 179-184. Please contact us again if you have any additional questions.