Overview

  • Product name
    Anti-Interferon alpha 1 antibody [F18]
    See all Interferon alpha 1 primary antibodies
  • Description
    Rat monoclonal [F18] to Interferon alpha 1
  • Host species
    Rat
  • Specificity
    This monoclonal antibody binds and neutralizes both natural and recombinant mouse alpha Interferon 1. This antibody does not cross-react with Human Interferon, Mouse Interferon beta or Mouse Interferon gamma.
  • Tested applications
    Suitable for: Flow Cyt, Neutralising, Other, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Positive control
    • Plasmacytoid dendritic cells.
  • General notes


    With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    0.2 µm filtered antibody solution.
  • Primary antibody notes
    With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.
  • Clonality
    Monoclonal
  • Clone number
    F18
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21172865

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Neutralising Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration. Useful for inhiiton in biological assays and for affinity pruification.

Target

  • Function
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFL antibody
    • IFN alpha antibody
    • IFN alpha 1/13 antibody
    • IFN alpha 1b antibody
    • IFN alphaD antibody
    • IFN antibody
    • IFN-alpha-1/13 antibody
    • IFNA@ antibody
    • IFNA1 antibody
    • IFNA1_HUMAN antibody
    • IFNA13 antibody
    • Interferon alpha 1 antibody
    • Interferon alpha-1/13 antibody
    • Interferon alpha-D antibody
    • Interferon alpha1 antibody
    • Interferon, alpha 13 antibody
    • LeIF D antibody
    see all

References

This product has been referenced in:
  • Biswas I  et al. Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-?-STAT1 signaling pathway. Eur J Immunol 45:3158-73 (2015). Read more (PubMed: 26350442) »
  • Björck P  et al. Plasmacytoid dendritic cell dichotomy: identification of IFN-a producing cells as a phenotypically and functionally distinct subset. J Immunol 186:1477-85 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21172865) »
See all 3 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that ab27715 is sourced externally and regrettably we are not able to provide the immunogen sequence. The source considers this to be proprietary information on this occasion. However, they have confirmed that the antibody will recognize all the ˜ 19 kDa murine IFN-α.

As far as we are aware, ab7373 has never been tested in western blot. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test ab7373 in WB, please contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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Answer

Thank you for your inquiry.

Yes, I can confirm that GRxx-2 and xx are both the same lot. These are just two different names for the same.I am sorry for this confusion and willforward this feedback.

Indeed, we added the GR lot numbers also for easier tracability - the number after the "-" indicates the packaging. So all lots GR39605 will come from the same production, the -2 one has been packed in a second round.

I hope this makes sense. Please do not hesitate to get back to us should you have any additional question or concern.

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Answer

Thank you for your message. If you have not already done so, you can place an order by calling us on +44(0)1223 696000 or email orders@abcam.com. Good luck with your research!

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Question
Answer

The antibody has been raised against purified natural L-cell IFN-alpha. The antibody reacts with alpha-1, alpha-2, alpha-4, alpha-5 (P), alpha-6 (T) and alpha-A subtypes.

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Question
Answer

Thank you for your enquiry. Ab7373 is only available in the form described on the online datasheet and we cannot do specialized orders.

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Answer

Thank you for your enquiry. Ab7373 has so far only been tested for application in Immunoprecipitation, Affinity purification, Immunoassay, and Inhibition Assay. Our mouse monoclonal Interferon alpha 2b antibodies - ab9386, ab9388, and ab5258 - all have all been tested for application in ELISA. If you have any more questions, please contact us again.

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Answer

Any rat IgG1 monoclonal antibody which doesn't bind to IFN-alpha can be useful as control.

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Answer

The antibody recognizes natural IFN-alpha from L-cells and several recombinant IFN-alpha subtypes. Neutralization of alpha-1, alpha-2, alpha-4, alpha-5 (P), alpha-6 (T) and alpha-A subtypes is possible.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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