Recombinant Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free (ab230830)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19074] to Interferon alpha 2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, ICC
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Interferon alpha 2 antibody [EPR19074] - BSA and Azide free
See all Interferon alpha 2 primary antibodies -
Description
Rabbit monoclonal [EPR19074] to Interferon alpha 2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: HEK-293T cells transfected with Interferon alpha 2 expression vector containing a GFP-Myc-tag / GFP-tag.
-
General notes
ab230830 is the carrier-free version of ab196221.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19074 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
ICC |
Use at an assay dependent concentration.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
Target
-
Function
Produced by macrophages, IFN-alpha have antiviral activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
-
Alternative names
- Alpha 2a interferon antibody
- IFN alpha 2 antibody
- IFN alphaA antibody
see all
Images
-
Interferon alpha 2 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate using ab196221 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab196221 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as for detection at 1/10000 dilution.
Lane 1: HEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate 10 μg (Input).
Lane 2: ab196221 IP in HEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196221 inHEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.The molecular mass observed is due to the presence of the GFP epitope tag on full length IFN alpha 2.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).
-
Intracellular flow cytometric analysis of�4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag cell line) cells labeling �Interferon alpha 2 with ab196221 at 1/600 (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor� 647) (ab150079), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).
-
Immunocytochemical analysis of agarose-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 (pcDNA3.1 (+)-EGFP-Myc)) cells labeling Interferon alpha 2 with ab196221 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use secondary antibody. Positive staining on HEK-293T cells transfected with Interferon alpha 2 (pcDNA3.1 (+)-EGFP-Myc) (image A); and no staining on HEK-293T cells without transfection (image B). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of ab196221, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with either GFP-tagged Interferon alpha 2 expression vector or empty GFP expression vector) cells labeling Interferon alpha 2 with ab196221 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged Interferon alpha 2 expression vector.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of ab196221, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196221).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab230830 has not yet been referenced specifically in any publications.