• Product name
    Anti-Interferon alpha 2b antibody [4E10]
    See all Interferon alpha 2b primary antibodies
  • Description
    Mouse monoclonal [4E10] to Interferon alpha 2b
  • Host species
  • Tested applications
    Suitable for: Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
  • General notes

    The neutralising bioassay was performed using L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the test antibodies for 2 h at RT and then added to the cell monolayer. 24 h later the cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. Polyclonal antibodies to respective interferons and irrelevant monoclonal antibody of the same isotype were used as controls.

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR317571-2, GR317571-3 and GR190761-4 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab9388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/500 - 1/2000.
WB 1/500.


  • Function
    Produced by macrophages, IFN-alpha have antiviral activities.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 2a interferon antibody
    • IFN alpha 2b antibody
    • IFN-alpha-2 antibody
    • IFNA antibody
    • Ifna2 antibody
    • IFNA2_HUMAN antibody
    • INFA2 antibody
    • Interferon alpha 2 antibody
    • Interferon alpha A antibody
    • Interferon alpha-2 antibody
    • Interferon alpha-A antibody
    • LeIF A antibody
    • LeIFA antibody
    see all


  • Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
    Lane 1 - interferon alpha 2b
    Lane 2 - prestained standard proteins.

    Immunoblot of human interferon alpha 2b using monoclonal antibody 4E10 at a dilution of 1:500 (4 µg/ml).
    Lane 1 - interferon alpha 2b
    Lane 2 - prestained standard proteins.


ab9388 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


Thank you for contacting us. Yes, these are the same lot numbers in all cases. The number after the - just indicates the time it was aliquotted, so if the GRXXXX is the same but the dash is different you can be assured the lot is still the same. Please let me know if you have any further questions.

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Thank you for contacting us.

We have used this antibody under reducing and denaturing conditions in western blot. We unfortunately haven't done any comparison studies between native vs reducing conditions. I am sorry we are unable to comment on this due to lack of tested data.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us. Concentrations of the antibodies ab9386 and ab9388 are determined spectrophotometrically by an adsorbance measurement at 280 nm. The absorbance value (A280) is divided by the extiction coefficient of mouse IgG - 1.35. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting Abcam. For both ab9386 and ab9388, we currently sell them in 100ul aliquots, at a concentration of 2mg/ml. From what I can see, there are no plans to change this concentration for future lots of the antibody and since it is a mouse monoclonal there should not be a large variation in batch to batch concentrations. If any changes do occur then the new concentration will be clearly displayed on the datasheet and/or vial. Please let me know if there is anything else I can help you with.

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Thank you for your enquiry. We currently do not have an antibody to human interferon alpha that is biotinylated. We have the following human interferon alpha antibodies: ab9660, ab9386, ab8317, ab9388, and ab5258 (HRP conjugated). If you have any more questions, please contact us again.

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To use monoclonal antibodies 9D3 and 4E10 in sandwich ELISA for the detection of huIFN-alpha-2b, 4E10 antibody should be labeled either with horse-radish peroxidase or biotin. Monoclonal antibody 9D3 should be used as a capture antibody (for plate coating). Before labeling procedure, antibody 4E10 should be dialysed to remove sodium azide (see Abcam protocol Azide Removal by Dialysis). Biotinylation protocols are available on-line (see, for example: www.protocol-online.org or www.drmr.com). Biotinylated 4E10 can be detected using Streptavidin-Peroxidase (widely used reagent, see, for example www.chemicom.com or www.sigmaaldrich.com). Labeling of monoclonal antibody with peroxidase is more complicated procedure than biotinylation (see, for example, HRP Conjugation Kit description at www.4adi.com). After preparation of 4E10 conjugate, it can be used together with 9D3 in sandwich ELISA for the detection of huIFN-alpha-2b. Short protocol is given below: 1. Plate coating: 9D3, 5 mcg/ml in Na-carbonate buffer (pH 9.5), 100 mcl/well, incubation overnight at +4 C; 2. Plate blocking: 2% BSA in PBS, 100 mcl/well, 1 h at RT; 3. Washing: 3 times with PBST (PBS with 0.1% Tween-20); 4. Incubation with IFN standard and test samples: 1 h at RT, 100 mcl/well, dilution buffer – PBST; 5. Washing: 5-6 times with PBST; 6. Incubation with 4E10 conjugate: 1 h at RT, dilution buffer – PBST. If 4E10-biotin is used, further incubation step with Streptavidin-Peroxidase is required; 7. Washing: 8-10 times with PBST; 8. Development of enzymatic reaction using TMB-substrate.

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