HRP Anti-Interferon alpha 2b antibody [4E10] (ab5258)
Key features and details
- HRP Mouse monoclonal [4E10] to Interferon alpha 2b
- Suitable for: ELISA
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
HRP Anti-Interferon alpha 2b antibody [4E10]
See all Interferon alpha 2b primary antibodies -
Description
HRP Mouse monoclonal [4E10] to Interferon alpha 2b -
Host species
Mouse -
Conjugation
HRP -
Specificity
Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Interferon alpha 2b.
-
General notes
Recommended ELISA procedure for human IFN alpha 2b determination: 1. Use 96-well Nunc Maxisorp plates for the assay. Coat microwells with 100 µl/well of antibody 9D3 (ab9386) diluted to 10 µg/ml in coating buffer (0.05 M Na-carbonate, pH 9.5). Incubate overnight at +4°C. 2. Aspirate wells and block with 150 µl/well of blocking buffer (2% BSA in PBS). Incubate at RT for 1 h. 3. Aspirate wells and wash 3 times with PBST (0.1% Tween-20 in PBS). 4. Prepare standard and sample dilutions in PBST. Recommended IFN alpha 2b concentrations: from 100 pg/ml to 100 ng/ml. Add 100 µl of each standard and sample into appropriate wells. Incubate at RT for 2 h. 5. Aspirate and wash 5 times with PBST. 6. Dilute detection antibody 4E10-HRP (ab5258) 1:1000 in PBST. Add 100 µl of diluted detection antibody to each well. Incubate at RT for 1 h. 7. Aspirate and wash 10 times with PBST. 8. Add 100 µl of substrate solution (TMB) to each well. Incubate at RT for 10-20 min. 9. Add 50 µl of stop solution (2 N H2SO4) to each well. Read absorbance at 450 nm.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR308991-1 and GR308991-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4E10 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Recombinant Protein
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. |
Target
-
Function
Produced by macrophages, IFN-alpha have antiviral activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
-
Alternative names
- Alpha 2a interferon antibody
- IFN alpha 2b antibody
- IFN-alpha-2 antibody
see all
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab5258 has been referenced in 2 publications.
- Chen J et al. Dissolving microneedle-based intradermal delivery of interferon-a-2b. Drug Dev Ind Pharm 42:890-6 (2016). PubMed: 26467418
- Podobnik B et al. Conjugation of PolyPEG to interferon alpha extends serum half-life while maintaining low viscosity of the conjugate. Bioconjug Chem 26:452-9 (2015). PubMed: 25629733