Recombinant
RabMAb

Recombinant Anti-Interferon alpha 6 antibody [EPR16355] (ab192264)

Overview

  • Product name

    Anti-Interferon alpha 6 antibody [EPR16355]
    See all Interferon alpha 6 primary antibodies
  • Description

    Rabbit monoclonal [EPR16355] to Interferon alpha 6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Interferon alpha 6 aa 50-150. The exact sequence is proprietary.
    Database link: P05013

  • Positive control

    • U937, human fetal liver and human fetal lung lysates: Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/50.
Flow Cyt 1/600.

Target

  • Relevance

    Produced by macrophages, IFN alpha have mainly antiviral activities.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • IFN alpha 6 antibody
    • IFN alphaK antibody
    • IFNA6 antibody
    • Interferon alpha 54 antibody
    • Interferon alpha K antibody
    • Interferon, alpha 6 antibody
    • LeIF K antibody
    see all

Images

  • All lanes : Anti-Interferon alpha 6 antibody [EPR16355] (ab192264) at 1/5000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal lung lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa



    Blocking/dilution buffer: 5% NFDM /TBST

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Interferon alpha 6 with ab192264 at 1/50 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green).  The nuclear counter stain is DAPI (blue). 

    The negative controls are as follows;
    1. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
    2. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.

  • Flow cytometry analysis of Jurkat cells labelling Interferon alpha 6 (red) with purified ab192264 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab192264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192264.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up