Product nameAnti-Interferon alpha 6 antibody [EPR16355]
See all Interferon alpha 6 primary antibodies
DescriptionRabbit monoclonal [EPR16355] to Interferon alpha 6
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Interferon alpha 6 aa 50-150. The exact sequence is proprietary.
Database link: P05013
- U937, human fetal liver and human fetal lung lysates: Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab192264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).|
RelevanceProduced by macrophages, IFN alpha have mainly antiviral activities.
- IFN alpha 6 antibody
- IFN alphaK antibody
- IFNA6 antibody
All lanes : Anti-Interferon alpha 6 antibody [EPR16355] (ab192264) at 1/5000 dilution
Lane 1 : U937 cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Blocking/dilution buffer: 5% NFDM /TBST
Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Interferon alpha 6 with ab192264 at 1/50 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
The negative controls are as follows;
1. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
2. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
Flow cytometry analysis of Jurkat cells labelling Interferon alpha 6 (red) with purified ab192264 at dilution of 1/600. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
ab192264 has not yet been referenced specifically in any publications.