Product nameAnti-Interferon alpha/beta receptor 1 antibody [EP899Y]
See all Interferon alpha/beta receptor 1 primary antibodies
DescriptionRabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
SpecificityThis antibody is specific for the 90kda form and the full-length form of Interferon alpha/beta receptor 1 protein.
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 500-600 (C terminal). The exact sequence is proprietary.
- WB: HeLa and K562 cell lysates. Flow cyt: K562 cells.
We recommend ab124764 as a better alternative to ab45172.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab45172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAssociates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.
Tissue specificityIFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
Sequence similaritiesBelongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains.
modificationsPhosphorylated on tyrosine residues by TYK2 tyrosine kinase.
- Information by UniProt
- Alpha type antiviral protein antibody
- Antiviral protein, alpha-type antibody
- Antiviral protein, beta-type antibody
All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/1000 dilution
Lane 1 : Wild-type (HeLa) whole cell lysate
Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout (HeLa) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 64 kDa
Lanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab45172 was shown to recognize Interferon alpha/beta receptor 1 in wild-type WT (HeLa) cells as signal was lost at the expected MW in IFNAR1 (Interferon Receptor alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab45172 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).
Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg
Goat anti rabbit HRP labelled. at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 130,90 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Xu W et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). Read more (PubMed: 30185588) »
- Cheng M et al. Interferon down-regulation of miR-1225-3p as an antiviral mechanism through modulating Grb2-associated binding protein 3 expression. J Biol Chem 293:5975-5986 (2018). Read more (PubMed: 29496996) »