Validated using a knockout cell line

Recombinant Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)


  • Product name

    Anti-Interferon alpha/beta receptor 1 antibody [EP899Y]
    See all Interferon alpha/beta receptor 1 primary antibodies
  • Description

    Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
  • Host species

  • Specificity

    This antibody is specific for the 90kda form and the full-length form of Interferon alpha/beta receptor 1 protein.
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and K562 cell lysates. Flow cyt: K562 cells.
  • General notes

    We recommend ab124764 as a better alternative to ab45172.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab45172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.
    • Tissue specificity

      IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
    • Sequence similarities

      Belongs to the type II cytokine receptor family.
      Contains 3 fibronectin type-III domains.
    • Post-translational

      Phosphorylated on tyrosine residues by TYK2 tyrosine kinase.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha type antiviral protein antibody
      • Antiviral protein, alpha-type antibody
      • Antiviral protein, beta-type antibody
      • AVP antibody
      • Beta type antiviral protein antibody
      • CRF2-1 antibody
      • Cytokine receptor class-II member 1 antibody
      • Cytokine receptor family 2 member 1 antibody
      • IFN alpha REC antibody
      • IFN alpha receptor antibody
      • IFN alpha/beta Receptor alpha antibody
      • IFN beta receptor antibody
      • IFN Interferon-beta receptor antibody
      • IFN-alpha/beta receptor 1 antibody
      • IFN-R-1 antibody
      • IFNAR antibody
      • Ifnar1 antibody
      • IFNBR antibody
      • IFRC antibody
      • INAR1_HUMAN antibody
      • Interferon (alpha beta and omega) receptor 1 antibody
      • interferon alpha and beta receptor subunit 1 antibody
      • Interferon alpha/beta receptor 1 antibody
      • Interferon alpha/beta receptor alpha chain antibody
      • Interferon beta receptor 1 antibody
      • interferon receptor 1 antibody
      • Interferon-alpha receptor antibody
      • Type I interferon receptor 1 antibody
      see all


    • All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/1000 dilution

      Lane 1 : Wild-type (HeLa) whole cell lysate
      Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout (HeLa) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 64 kDa

      Lanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab45172 was shown to recognize Interferon alpha/beta receptor 1 in wild-type WT (HeLa) cells as signal was lost at the expected MW in IFNAR1 (Interferon Receptor alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab45172 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).
    • Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg

      Goat anti rabbit HRP labelled. at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 130,90 kDa
      why is the actual band size different from the predicted?


    This product has been referenced in:

    • Xu W  et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). Read more (PubMed: 30185588) »
    • Cheng M  et al. Interferon down-regulation of miR-1225-3p as an antiviral mechanism through modulating Grb2-associated binding protein 3 expression. J Biol Chem 293:5975-5986 (2018). Read more (PubMed: 29496996) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Flow Cytometry
    BD #554722
    Human Cell (primary human macrophages)
    primary human macrophages
    Gating Strategy

    Abcam user community

    Verified customer

    Submitted Jul 19 2013


    Thank you for contacting us.

    I would recommend you consult the manufacturer of the beads which you intent to use. The binding capacity will be determined by the manufacturer of the beads. For example Millipore'sPureProteome Protein A and Protein G Magnetic Beadshave a binding capacity of2.5–3.5 µg of Rabbit IgG per µL of suspension. While NEB'sProtein G Magnetic Beads have a binding capacity of 400µg/µl of Human IgG.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!

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    Western blot
    Human Cell lysate - whole cell (vascular smooth muscle cells)
    Loading amount
    10 µg
    vascular smooth muscle cells
    DTT and 5 min boiling
    Gel Running Conditions
    Reduced Denaturing (4-12 % gradients)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Apr 27 2012


    Thank you for your enquiry. A general protocol for intracellular staining with this antibody can be found at the following link: A second protocol describes indirect staining which can be adapted to intracellular staining: For a secondary antibody, scroll to the bottom of the online datasheet for ab45172 where you will find a list of compatible secondary antibodies. For an isotype control, use normal rabbit IgG, for instance ab37415. The concentration of ab45172 is 0.15 mg/ml.

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