Recombinant Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-324331-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-western-blot.jpg "Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Interferon alpha/beta receptor 1 antibody [EP899Y]
See all Interferon alpha/beta receptor 1 primary antibodies -
Description
Rabbit monoclonal [EP899Y] to Interferon alpha/beta receptor 1 -
Host species
Rabbit -
Specificity
This antibody is specific for the 90kda form and the full-length form of Interferon alpha/beta receptor 1 protein. -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 500-600 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa and K562 cell lysates. Flow cyt: K562 cells. IP: K-562 cell lysate
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General notes
We recommend ab124764 as a better alternative to ab45172.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP899Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab45172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species. | |
| Flow Cyt | 1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/50. |
Target
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Function
Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. -
Tissue specificity
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by TYK2 tyrosine kinase. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3454 Human
- Omim: 107450 Human
- SwissProt: P17181 Human
- Unigene: 529400 Human
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Alternative names
- Alpha type antiviral protein antibody
- Antiviral protein, alpha-type antibody
- Antiviral protein, beta-type antibody
see all
Images
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Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/1000 dilution
Lane 1 : Wild-type (HeLa) whole cell lysate
Lane 2 : IFNAR1 (Interferon Receptor alpha) knockout (HeLa) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 64 kDaLanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 64 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab45172 was shown to recognize Interferon alpha/beta receptor 1 in wild-type WT (HeLa) cells as signal was lost at the expected MW in IFNAR1 (Interferon Receptor alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 (Interferon Receptor alpha) knockout samples were subjected to SDS-PAGE. Ab45172 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-7-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-immunoprecipitation-k562-human.jpg)
Immunoprecipitation - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)Purified ab45172 at 1/50 dilution (2µg) immunoprecipitating Interferon alpha/beta receptor 1 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab45172 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45172 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 130 kDa -
![Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-57337-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-flow-cytometry.jpg)
Flow Cytometry - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green). -
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-57338-anti-interferon-alpha-beta-receptor-1-antibody-ep899y-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg
Secondary
Goat anti rabbit HRP labelled. at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 130,90 kDa why is the actual band size different from the predicted? -
![Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)](https://www.abcam.com/ps/products/45/ab45172/Images/ab45172-6-benefits-of-recombinant-antibodies.png)
Anti-Interferon alpha/beta receptor 1 antibody [EP899Y] (ab45172)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (19)
ab45172 has been referenced in 19 publications.
- Chen T et al. Involvement of androgen receptor (AR)/microRNA-21 axis in hypoxia/reoxygenation-induced apoptosis of mouse renal tubular epithelial cells. Am J Transl Res 11:5611-5622 (2019). PubMed: 31632533
- Read SA et al. Macrophage Coordination of the Interferon Lambda Immune Response. Front Immunol 10:2674 (2019). PubMed: 31798594
- Ortiz A et al. An Interferon-Driven Oxysterol-Based Defense against Tumor-Derived Extracellular Vesicles. Cancer Cell 35:33-45.e6 (2019). PubMed: 30645975
- Xu W et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol 92:N/A (2018). PubMed: 30185588
- Cheng M et al. Interferon down-regulation of miR-1225-3p as an antiviral mechanism through modulating Grb2-associated binding protein 3 expression. J Biol Chem 293:5975-5986 (2018). PubMed: 29496996
