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I am also interested in cross linking the antibody to protein G magnetic beads. Is there a specific amount of antibody that can be cross-linked per volume of beads?
Asked on Jun 04 2012
Thank you for contacting us.
I would recommend you consult the manufacturer of the beads which you intent to use. The binding capacity will be determined by the manufacturer of the beads. For example Millipore'sPureProteome Protein A and Protein G Magnetic Beadshave a binding capacity of2.5–3.5 µg of Rabbit IgG per µL of suspension. While NEB'sProtein G Magnetic Beads have a binding capacity of 400µg/µl of Human IgG.
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Answered on Jun 04 2012