Recombinant Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
Anti-Interferon alpha/beta receptor 1 antibody [EPR6244]
See all Interferon alpha/beta receptor 1 primary antibodies -
Description
Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 -
Host species
Rabbit -
Specificity
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Interferon alpha/beta receptor 1 aa 100-200. The exact sequence is proprietary.
Database link: P17181 -
Positive control
- HeLa, HeLa treated with IFN-alpha, K562 and U937 cell lysates.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6244 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa). |
Target
-
Function
Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. -
Tissue specificity
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by TYK2 tyrosine kinase. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3454 Human
- Omim: 107450 Human
- SwissProt: P17181 Human
- Unigene: 529400 Human
-
Alternative names
- Alpha type antiviral protein antibody
- Antiviral protein, alpha-type antibody
- Antiviral protein, beta-type antibody
see all
Images
-
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.com/ps/products/124/ab124764/Images/ab124764-310945-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : Interferon alpha/beta receptor 1 knockout HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 64 kDaLanes 1 - 3: Merged signal (red and green). Green - ab124764 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab124764 was shown to specifically react with Interferon Receptor alpha in wild-type HeLa cells as signal was lost in Interferon alpha/beta receptor 1 knockout cells. Wild-type and Interferon alpha/beta receptor 1 knockout samples were subjected to SDS-PAGE. Ab124764 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.com/ps/products/124/ab124764/Images/ab124764-257152-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 (ab124764) at 1/1000 dilution
Predicted band size: 64 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTIn mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
-
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.com/ps/products/124/ab124764/Images/ab124764-257151-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)
Lane 1 : HEK293 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lane 4 : SH-SY5Y whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.com/ps/products/124/ab124764/Images/ab124764-256793-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain whole cell lysate
Lane 2 : Mouse heart whole cell lysate
Lane 3 : Mouse kidney whole cell lysate
Lane 4 : Mouse spleen whole cell lysate
Lane 5 : Rat brain whole cell lysate
Lane 6 : Rat heart whole cell lysate
Lane 7 : Rat kidney whole cell lysate
Lane 8 : Rat spleen whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated. at 1/2000 dilution
Predicted band size: 64 kDa
Exposure time: 3 minutesBlocking buffer: 5% NFDM/TBST.
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
-
![Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.com/ps/products/124/ab124764/Images/ab124764-138612-anti-interferon-alpha-beta-receptor-1-antibody-epr6244-western-blot.jpg)
Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with IFN-alpha
Lane 3 : K562 cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/200 dilution
Predicted band size: 64 kDa -
![Other - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)](https://www.abcam.com/ps/products/124/ab124764/Images/ab124764-138611-anti-interferon-alpha-beta-receptor-1-antibody-epr6244.jpg)
Other - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sen A et al. Rotavirus degrades multiple type interferon receptors to inhibit IFN signaling and protects against mortality from endotoxin in suckling mice. J Virol N/A:N/A (2017). Read more (PubMed: 29070687) »
- Chmiest D et al. Spatiotemporal control of interferon-induced JAK/STAT signalling and gene transcription by the retromer complex. Nat Commun 7:13476 (2016). Read more (PubMed: 27917878) »
