Anti-Interferon beta antibody [7F-D3] (ab24324)


  • Product name
    Anti-Interferon beta antibody [7F-D3]
    See all Interferon beta primary antibodies
  • Description
    Rat monoclonal [7F-D3] to Interferon beta
  • Host species
  • Specificity
    This antibody cross react with IFN beta, but not alpha or gamma.
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Purified mouse interferon beta.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 



Our Abpromise guarantee covers the use of ab24324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml. PubMed: 24474800

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.


Neutralising Use at an assay dependent concentration. At a concentration of 3.6 ng/ml, this antibody neutralizes 10 U/ml of mouse IFN-b to 1 U/ml.


  • Function
    Has antiviral, antibacterial and anticancer activities.
  • Sequence similarities
    Belongs to the alpha/beta interferon family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • beta-interferon antibody
    • Fibroblast interferon antibody
    • IFB antibody
    • IFF antibody
    • IFN beta antibody
    • IFN-beta antibody
    • IFNB 1 antibody
    • IFNB antibody
    • IFNB_HUMAN antibody
    • IFNB1 antibody
    • Interferon beta 1 fibroblast antibody
    • Interferon beta antibody
    • Interferon beta precursor antibody
    • MGC96956 antibody
    see all


This product has been referenced in:
  • Wu H  et al. Mouse Testicular Cell Type-Specific Antiviral Response against Mumps Virus Replication. Front Immunol 8:117 (2017). WB ; Mouse . Read more (PubMed: 28239382) »
  • Hou C  et al. Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. Int J Mol Med 38:713-20 (2016). WB ; Mouse . Read more (PubMed: 27460435) »

See all 9 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Recombinant protein (recombinant IFNbeta AND mouse hepatocyte lysate)
Loading amount
0.5 µg
recombinant IFNbeta AND mouse hepatocyte lysate
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
50:50 PBS:Odyssey Blocking Buffer, Li-Cor 927-40100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 28 2012

Thank you for your reply. I am very glad to be of help with this. You understand the program perfectly. I have created a code for testing Ab24324 in WB for you below. There is a 4 month window for our 100% Abreview Testing Program within which you m...

Read More

Thank you for your reply. This clone was first developed and referenced in 1980 by Kawade, Y. J. Interferon Res. 1, 61-70 (1980). As far as I am aware it was created using purified mouse IFNB and not recombinant protein. However I do not have access...

Read More

Thank you for your reply.

I do understand your concerns. It may be that you would want to run a western blot with this product to ensure that it is picking up IFN from your cells, it would them be possible to run a purified protein as control...

Read More

Thank you for contacting us.

The name of the hybridoma cell line is 7F-D3 - the same as the clone number.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. ...

Read More

Thank you for contacting us. I am sorry that the two vials you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of *****. ...

Read More

Thank you for contacting us. We have tested this antibody in indirect ELISA and neutralization assays only. We haven’t tested this antibody in sELISA in combination with other antibodies so we will not be able to recommend the suitable ELISA pair f...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (eye tissue)
eye tissue
Antigen retrieval step
Heat mediated

Abcam user community

Verified customer

Submitted Apr 20 2011


Sign up